Human Notch 2 Protein (NOT-HM402)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Leu26-GIn530

Catalog: NOT-HM402

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Product Description Recombinant Human Notch 2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu26-GIn530.

Product Background

 Human Notch 2 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes. Although Notch proteins are structurally and functionally similar, deletion of either Notch-1 or Notch 2 is lethal, showing that not all functions overlap.Notch 2 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus.

Product Category

 Recombinant Protein

Target

 Notch 2
Synonyms AGS2; Notch-2; NOTCH2; N2ECD; HJCYS; N2
Accession

Species

 Human

Amino Acid Range

 Leu26-GIn530
Molecular Weight The protein has a predicted MW of 57 kDa. Due to glycosylation, the protein migrates to 68-78 kDa based on Tris-Bis PAGE result.

Product Tag

 C-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Human Notch 2 is greater than 95% as determined by SEC-HPLC.
Human Notch 2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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