Human Notch 2 Protein (NOT-HM402)
Human Notch 2 Protein (NOT-HM402)
Human Notch 2 Protein (NOT-HM402)
Human Notch 2 Protein (NOT-HM402)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Leu26-GIn530
Catalog #NOT-HM402
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Product Description
 Recombinant Human Notch 2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu26-GIn530.
Product Background
 Human Notch 2 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes. Although Notch proteins are structurally and functionally similar, deletion of either Notch-1 or Notch 2 is lethal, showing that not all functions overlap.Notch 2 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus.
Product Category
 Recombinant Protein
Target
 Notch 2
Synonyms
 AGS2; Notch-2; NOTCH2; N2ECD; HJCYS; N2
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu26-GIn530
Molecular Weight
 The protein has a predicted MW of 57 kDa. Due to glycosylation, the protein migrates to 68-78 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Notch 2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Notch 2 is greater than 95% as determined by SEC-HPLC.
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