Human Notch 2 Protein (NOT-HM402)
Human Notch 2 Protein (NOT-HM402)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Leu26-GIn530
Catalog #NOT-HM402
Regular price $150.00
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Product Description
Recombinant Human Notch 2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu26-GIn530.
Product Background
Human Notch 2 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes. Although Notch proteins are structurally and functionally similar, deletion of either Notch-1 or Notch 2 is lethal, showing that not all functions overlap.Notch 2 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus.
Product Category
Recombinant Protein
Target
Notch 2
Synonyms
AGS2; Notch-2; NOTCH2; N2ECD; HJCYS; N2
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Leu26-GIn530
Molecular Weight
The protein has a predicted MW of 57 kDa. Due to glycosylation, the protein migrates to 68-78 kDa based on Bis-Tris PAGE result.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Notch 2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Notch 2 is greater than 95% as determined by SEC-HPLC.

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