Product Description |
Recombinant Human OSMR&GP130 Protein is expressed from HEK293 with His tag and Flag tag at the C-Terminus.It contains Glu28-Met740(OSMR)&Glu23-Glu619(GP130). |
Product Background |
OSMR&gp130 is a type II receptor for OSM in humans. OSM can alternatively bind to a heterodimer of gp130 and LIF-R or of gp130 and OSMR (OSM receptor). On the basis of in vitro studies, the OSM ligand recruits a broader array of cell signaling pathways than most gp130 cytokines. OSMR and gp130 chains are widely expressed in connective tissue cells and tumor cells. |
Product Category |
Recombinant Protein |
Target |
OSMR&GP130 |
Synonyms |
OSMR&GP130; OSMR; GP130; IL-31 receptor subunit beta&GP130; IL-31R subunit beta&GP130; IL-31R-beta&GP130; IL-31RB&GP130; OSMRB&GP130 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu28-Met740(OSMR) & Glu23-Glu619(GP130) |
Molecular Weight |
The protein has a predicted MW of 82.2 kDa(OSMR) and 68.83 kDa(GP130). Due to glycosylation, the protein migrates to 100-140 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human OSMR&GP130, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His, C-Flag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human OSMR&GP130, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA. |
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Human OSMR&GP130 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human OSMR&GP130 is greater than 95% as determined by SEC-HPLC. |
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