Human OSMR&GP130 Protein (OGC-HM10R)

  • Express System: Expi293
  • Product Tag: C-His-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human OSMR&GP130, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Glu28-Met740(OSMR) & Glu23-Glu619(GP130)

Catalog: OGC-HM10R

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Product Description Recombinant Human OSMR&GP130 Protein is expressed from Expi293 with His tag and Flag tag at the C-terminal.It contains Glu28-Met740(OSMR)&Glu23-Glu619(GP130).
Product Category Recombinant Protein
Target OSMR&GP130
Gene/Protein ID Q99650-1(OSMR)&P40189-1(GP130)
Species Human
Exact Sequence Glu28-Met740(OSMR) & Glu23-Glu619(GP130)
Molecular Weight The protein has a predicted MW of 82.2 kDa(OSMR) and 68.83 kDa(GP130). Due to glycosylation, the protein migrates to 100-140 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human OSMR&GP130, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Flag
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human OSMR&GP130, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA.
The purity of Human OSMR&GP130 is greater than 95% as determined by SEC-HPLC.
Human OSMR&GP130 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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