Name: Human PAI-1 Protein (PAI-HM101)
Brand: KactusBio
SKU: PAI-HM101-100UG
Price: 264.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The IC50 value is < 35 nM.
Amino Acid Range: Val24-Pro402
Other Available Proteins:

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Catalog #PAI-HM101

Size

100UG

500UG

Order by 4pm ET for delivery on:

Human PAI-1 Protein (PAI-HM101) Summary

Product Description	Recombinant Human PAI-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val24-Pro402.
Product Background	PAI-1 (plasminogen activator inhibitor-1) is a member of plasminogen cascade with an inhibitory role in plasmin activation. PAI-1 is an important regulator of the fibrinolytic process and levels of this antifibrinolytic protein are elevated in diabetes and insulin resistant states.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human PAI-1 Protein (PAI-HM101) Specifications/Details

Protein	PAI-1
Synonyms	PAI; PAI-1; Serpin E1; SERPINE1; PAI1; PLANH1
Accession	P05121-1
Species	Human
Biotinylated	no
Amino Acid Range	Val24-Pro402
Molecular Weight	The protein has a predicted MW of 43.86 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
Activity	Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The IC50 value is < 35 nM.
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50mM NaAC, 0.1M NaCl, 100mM L-arginine (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 50mM NaAC (pH 5.5). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human PAI-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human PAI-1 is greater than 95% as determined by SEC-HPLC.

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