• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Met1-His118
Catalog #SGR-HM201
Price
Regular price $26400
Regular price Sale price $26400
/
Size
Avantor VWR Fisher Scientific

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Human SIGIRR Protein (SGR-HM201) Summary

Product Description

 Recombinant Human SIGIRR Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Met1-His118.

Product Background

 TIR8, also known as single Ig IL-1-related receptor, is a member of the IL-1 receptor/Toll-like receptor (TLR) superfamily, which acts as an intracellular decoy for components of the signaling pathway. Structurally, it is characterized by a single extracellular Ig domain, an intracellular TIR domain and 95-aa cytoplasmic tail. Recently, TIR8 was shown to inhibit NF-B activation by members of the IL-1/TLR family. The inhibitory activity of TIR8 was associated with trapping of TRAF6 and IRAK1.

Human SIGIRR Protein (SGR-HM201) Specifications/Details

Protein
 SIGIRR
Synonyms
 TIR8; Single Ig IL-1-related receptor; IL-1R8;Single Ig IL1-related receptor; IL1R8
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Met1-His118
Molecular Weight
 The protein has a predicted MW of 39.29 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human SIGIRR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human SIGIRR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human SIGIRR is greater than 95% as determined by SEC-HPLC. The purity of Human SIGIRR is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...