SEC-HPLC chromatogram of protein sample showing a single sharp peak at retention time 6.945 minutes, indicating high purity.
Human VCAM-1/CD106 Protein (VAM-HM106)
SEC-HPLC chromatogram of protein sample showing a single sharp peak at retention time 6.945 minutes, indicating high purity.
Human VCAM-1/CD106 Protein (VAM-HM106)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Phe25-Pro697
  • Other Available Proteins:
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Catalog #VAM-HM106
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Size
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Human VCAM-1/CD106 Protein (VAM-HM106) Summary

Product Description

 Recombinant Human VCAM-1/CD106 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe25-Pro697.

Product Background

 Tumor necrosis factor alpha (TNFα) is a pro-inflammatory cytokine that triggers the expression of inflammatory molecules, including other cytokines and cell adhesion molecules. TNFα induces the expression of intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 (VCAM-1). VCAM-1 was originally identified as a cell adhesion molecule that helps regulate inflammation-associated vascular adhesion and the transendothelial migration of leukocytes, such as macrophages and T cells.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human VCAM-1/CD106 Protein (VAM-HM106) Specifications/Details

Protein
 VCAM-1/CD106
Synonyms
 V-CAM 1; VCAM-1; INCAM-100; CD106; DKFZp779G2333; MGC99561
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Phe25-Pro697
Molecular Weight
 The protein has a predicted MW of 75.2 kDa. Due to glycosylation, the protein migrates to 75-110 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human VCAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human VCAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human VCAM-1 is greater than 95% as determined by SEC-HPLC. The purity of Human VCAM-1 is greater than 95% as determined by SEC-HPLC.
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support@kactusbio.us

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