Dose-response curve showing the bioactivity of recombinant human Wnt surrogate-Fc fusion protein, with RFU increasing sigmoidally with protein concentration, demonstrating activation in HEK293T cells.
Human Wnt Surrogate-Fc Fusion Protein (WNT-HM23A)
Human Wnt Surrogate-Fc Fusion Protein (WNT-HM23A)
Human Wnt Surrogate-Fc Fusion Protein (WNT-HM23A)
Dose-response curve showing the bioactivity of recombinant human Wnt surrogate-Fc fusion protein, with RFU increasing sigmoidally with protein concentration, demonstrating activation in HEK293T cells.
Human Wnt Surrogate-Fc Fusion Protein (WNT-HM23A)
Human Wnt Surrogate-Fc Fusion Protein (WNT-HM23A)
Human Wnt Surrogate-Fc Fusion Protein (WNT-HM23A)
  • Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE
Catalog #WNT-HM23A
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Size
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Human Wnt Surrogate-Fc Fusion Protein (WNT-HM23A) Summary

Product Description

 Recombinant Human Wnt Surrogate-Fc Fusion Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.

Product Background

 Wnt-3a is regarded as an activator of the canonical Wnt signaling pathway. This activator is expressed in the dorsal midline region and is responsible for spinal cord development. In addition, Wnt-3a plays a regulatory role in autophagy, apoptosis, and regeneration of neurons, neurogenic inflammation and axon regeneration.Wnt-3a promotes the beta-catenin/Tcf pathway which is tumor inducing and can cause cancer when expressed in particular cell populations. It is also one of the most commonly used cytokines for organoid construction.

Product Category

 Recombinant Protein / Cell therapy / Cell culture / Cytokines

Human Wnt Surrogate-Fc Fusion Protein (WNT-HM23A) Specifications/Details

Protein
 Wnt Surrogate
Synonyms
 Wnt-3a; Wnt 3a; WNT3A protein; Wnt3a
Species
 Human
Biotinylated
 no
Molecular Weight
 The protein has a predicted MW of 58.50 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result.
Product Tag
 C-hFc (IgG1)

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human Wnt Surrogate-Fc Fusion on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Wnt Surrogate-Fc Fusion on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50-for this effect is 2-12 ng/mL (QC Test). Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50-for this effect is 2-12 ng/mL (QC Test).
Other Available Proteins:
Finding available proteins...
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