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Monkeypox virus A30 Protein (A30-VM130) Summary
Product Description |
Recombinant Monkeypox virus A30 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln22-Leu146. |
Product Background |
Monkeypox virus (MPXV) is double-stranded DNA virus belonging to the genus orthopoxvirus that causes a smallpox-like disease in humans. A30L is an envelope protein required for the fusion of virus and host cell to form syncytia, and is also considered to be an important target in MPXV research. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Monkeypox virus A30 Protein (A30-VM130) Specifications/Details
Protein |
Monkeypox virus/A30 |
Synonyms |
Envelope protein A28 homolog; Protein A30; A30L; A30 |
Accession |
|
Species |
Monkeypox virus |
Biotinylated |
no |
Amino Acid Range |
Gln22-Leu146 |
Molecular Weight |
The protein has a predicted MW of 15.24 kDa. Due to glycosylation, the protein migrates to 30-55 kDa based on Bis-Tris PAGE result. |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Monkeypox virus A30 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Monkeypox virus A30 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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