Product Description |
Recombinant Monkeypox virus A30 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln22-Leu146. |
Product Background |
Monkeypox virus (MPXV) is double-stranded DNA virus belonging to the genus orthopoxvirus that causes a smallpox-like disease in humans. A30L is an envelope protein required for the fusion of virus and host cell to form syncytia, and is also considered to be an important target in MPXV research. |
Product Category |
Recombinant Protein |
Target |
A30 |
Accession |
|
Species |
Monkeypox virus |
Biotinylated |
no |
Amino Acid Range |
Gln22-Leu146 |
Molecular Weight |
The protein has a predicted MW of 15.24 kDa. Due to glycosylation, the protein migrates to 30-55 kDa based on Bis-Tris PAGE result. |
Product Tag |
N-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Monkeypox virus A30 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Monkeypox virus A30 is greater than 95% as determined by SEC-HPLC. |
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