SEC-HPLC chromatogram of recombinant protein with a dominant peak at 7.886 minutes, indicating high monomer purity and minimal aggregation.
Mouse APLN Protein (APN-MM201)
Mouse APLN Protein (APN-MM201)
  • Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Amino Acid Range: Val23-Phe77
Catalog #APN-MM201
Regular price $297.00
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Size
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Mouse APLN Protein (APN-MM201) Summary

Product Description

Recombinant Mouse APLN Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Val23-Phe77.

Product Background

Macrophages play key roles during cardiovascular diseases (CVD) and their related complications.Apelin (APLN) is a key molecule, whose roles during CVD have been documented previously. Therefore, it has been hypothesized that APLN may perform its roles via modulation of macrophages. Additionally, due to the widespread distribution of the CVD, more effective therapeutic strategies need to be developed to overcome the related complications.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse APLN Protein (APN-MM201) Specifications/Details

Protein
APLN
Synonyms
Apelin;APJ endogenous ligand;APEL
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Val23-Phe77
Molecular Weight
The protein has a predicted MW of 33 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
Product Tag
C-hFc (IgG1)

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS, 200mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse APLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse APLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...
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