Mouse CD40 Ligand/TNFSF5 Trimer Protein (CDL-MM240)
Mouse CD40 Ligand/TNFSF5 Trimer Protein (CDL-MM240)
Mouse CD40 Ligand/TNFSF5 Trimer Protein (CDL-MM240)
Mouse CD40 Ligand/TNFSF5 Trimer Protein (CDL-MM240)
  • Express System: HEK293
  • Product Tag: N-monomeric hFc
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Mouse CD40, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Met112-Leu260
Catalog #CDL-MM240
Regular price $255.00
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Product Description
Recombinant Mouse CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus; It contains Met112-Leu260.
Product Background
CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
Product Category
Recombinant Protein
Target
CD40 Ligand/TNFSF5 Trimer
Synonyms
CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Met112-Leu260
Molecular Weight
The protein has a predicted MW of 75.6 kDa. Due to glycosylation, the protein migrates to 76-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse CD40, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA. See testing image for detail.
Product Tag
N-monomeric hFc
Express System
HEK293
Purity
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS, 200 mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse CD40, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA.
Mouse CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.

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