Mouse CD40 Ligand/TNFSF5 Trimer Protein (CDL-MM240)
Catalog: CDL-MM240
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Product Description | Recombinant Mouse CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus.It contains Met112-Leu260. |
Product Category | Recombinant Protein |
Target | CD40 Ligand/TNFSF5 Trimer |
Synonyms | CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand |
Gene/Protein ID | P27548 |
Species | Mouse |
Exact Sequence | Met112-Leu260 |
Molecular Weight | The protein has a predicted MW of 75.6 kDa. Due to glycosylation, the protein migrates to 76-80 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Mouse CD40, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA. See testing image for detail. |
Product Tag | N-monomeric hFc |
Express System | HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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