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Mouse CD40 Ligand/TNFSF5 Trimer Protein (CDL-MM240) Summary
Product Description |
Recombinant Mouse CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus; It contains Met112-Leu260 trimer design. |
Product Background |
CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Mouse CD40 Ligand/TNFSF5 Trimer Protein (CDL-MM240) Specifications/Details
Protein |
CD40 Ligand/TNFSF5 |
Synonyms |
CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Met112-Leu260 |
Molecular Weight |
The protein has a predicted MW of 75.6 kDa. Due to glycosylation, the protein migrates to 76-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse CD40, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA. |
Product Tag |
N-monomeric hFc |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 200 mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Mouse CD40, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA. |
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Mouse CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Mouse CD40, His Tag on Anti-His-Biosensor can bind Mouse CD40 Ligand (Trimer), monomeric hFc Tag with an affinity constant of 0.10 nM as determined in BLI assay . |
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The purity of Mouse CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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