Name: Mouse CD40 Ligand/TNFSF5 Trimer Protein (CDL-MM240)
Brand: KactusBio
SKU: CDL-MM240-100UG
Price: 255.0 USD
Availability: InStock

Mouse CD40 Ligand/TNFSF5 Trimer Protein (CDL-MM240)

Immobilized Mouse CD40, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA.

Mouse CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Mouse CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-monomeric hFc
Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Activity: Immobilized Mouse CD40, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Met112-Leu260

Catalog #CDL-MM240

Size

100UG

500UG

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Product Description	Recombinant Mouse CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus; It contains Met112-Leu260.
Product Background	CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
Product Category	Recombinant Protein / Antibody discovery

Protein	CD40 Ligand/TNFSF5 Trimer
Synonyms	CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
Accession	P27548
Species	Mouse
Biotinylated	no
Amino Acid Range	Met112-Leu260
Molecular Weight	The protein has a predicted MW of 75.6 kDa. Due to glycosylation, the protein migrates to 76-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse CD40, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-monomeric hFc
Expression System	HEK293
Purity	>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 200 mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Mouse CD40, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA.
	Mouse CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.