Mouse CD40 Ligand/TNFSF5 Trimer Protein (CDL-MM240)

  • Express System: Expi293
  • Product Tag: N-monomeric hFc
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Mouse CD40, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 78.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Met112-Leu260

Catalog: CDL-MM240

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Product Description Recombinant Mouse CD40 Ligand/TNFSF5 Trimer Protein is expressed from Expi293 with monomeric hFc tag at the N-terminal.It contains Met112-Leu260.
Product Category Recombinant Protein
Target CD40 Ligand/TNFSF5 Trimer
Synonyms CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
Gene/Protein ID P27548
Species Mouse
Exact Sequence Met112-Leu260
Molecular Weight The protein has a predicted MW of 75.6 kDa. Due to glycosylation, the protein migrates to 76-80 kDa based on Tris-Bis PAGE result.
Activity Immobilized Mouse CD40, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 78.3ng/ml determined by ELISA. See testing image for detail.
Product Tag N-monomeric hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse CD40, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 78.3ng/ml determined by ELISA.
Mouse CD40 Ligand Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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