Mouse CD40 Ligand/TNFSF5 Trimer Protein (CDL-MM240)

  • Express System: HEK293
  • Product Tag: N-monomeric hFc
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Mouse CD40, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 25.6 ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Met112-Leu260

Catalog: CDL-MM240

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Product Description Recombinant Mouse CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with monomeric hFc tag at the N-Terminus.; It contains Met112-Leu260.

Product Background

CD40 ligand or CD40L, also called CD154, is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.

Product Category

Recombinant Protein

Target

CD40 Ligand/TNFSF5 Trimer
Synonyms CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand

Gene/Protein ID

P27548

Species

Mouse

Exact Sequence

Met112-Leu260
Molecular Weight The protein has a predicted MW of 75.6 kDa. Due to glycosylation, the protein migrates to 76-80 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse CD40, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 25.6 ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-monomeric hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1 EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS, 200 mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse CD40, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 25.6 ng/ml determined by ELISA.
Mouse CD40 Ligand Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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