Mouse CPM Protein (CPM-MM101) Summary
Product Description |
Recombinant Mouse CPM Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu18-Ser423. |
Product Background |
Carboxypeptidase M (CPM) is a glycosylphosphatidylinositol anchored enzyme that plays an important role in the kallikrein-kinin system (KKS). CPM catalytic domain hydrolyzes Arg from C-terminal peptides (i.e., bradykinin and kallidin), generating des-Arg-kinins, the agonists of B1 receptor (B1R). |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse CPM Protein (CPM-MM101) Specifications/Details
Protein |
CPM |
Synonyms |
Carboxypeptidase M; 1110060I01Rik; AA589379 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Leu18-Ser423 |
Molecular Weight |
The protein has a predicted MW of 47.51 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result. |
Activity |
Measured by its ability to release L-arginine from Benzoyl-Ala-Arg, with detection of the arginine amino group by o-phthaldialdehyde. The specific activity is >40,000 pmol/min/ug. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Other Available Proteins:
Finding available proteins...
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