Mouse CPM Protein (CPM-MM101)

SKU: CPM-MM101-100UG

Mouse CPM Protein (CPM-MM101)

SKU: CPM-MM101-100UG
Mouse CPM Protein (CPM-MM101)
Mouse CPM Protein (CPM-MM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its ability to release L-arginine from Benzoyl-Ala-Arg, with detection of the arginine amino group by o-phthaldialdehyde. The specific activity is >40,000 pmol/min/ug.
  • Amino Acid Range: Leu18-Ser423
Catalog #CPM-MM101
Regular price $330.00
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Size
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Mouse CPM Protein (CPM-MM101) Summary

Product Description

Recombinant Mouse CPM Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu18-Ser423.

Product Background

Carboxypeptidase M (CPM) is a glycosylphosphatidylinositol anchored enzyme that plays an important role in the kallikrein-kinin system (KKS). CPM catalytic domain hydrolyzes Arg from C-terminal peptides (i.e., bradykinin and kallidin), generating des-Arg-kinins, the agonists of B1 receptor (B1R).

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse CPM Protein (CPM-MM101) Specifications/Details

Protein
CPM
Synonyms
Carboxypeptidase M; 1110060I01Rik; AA589379
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Leu18-Ser423
Molecular Weight
The protein has a predicted MW of 47.51 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
Activity
Measured by its ability to release L-arginine from Benzoyl-Ala-Arg, with detection of the arginine amino group by o-phthaldialdehyde. The specific activity is >40,000 pmol/min/ug.
Product Tag
C-His

Fluorophore

Unconjugated
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse CPM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse CPM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse CPM is greater than 95% as determined by SEC-HPLC. The purity of Mouse CPM is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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