Product Description |
Recombinant Mouse CPM Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu18-Ser423. |
Product Background |
Carboxypeptidase M (CPM) is a glycosylphosphatidylinositol anchored enzyme that plays an important role in the kallikrein-kinin system (KKS). CPM catalytic domain hydrolyzes Arg from C-terminal peptides (i.e., bradykinin and kallidin), generating des-Arg-kinins, the agonists of B1 receptor (B1R). |
Product Category |
Recombinant Protein |
Target |
CPM |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Leu18-Ser423 |
Molecular Weight |
The protein has a predicted MW of 47.51 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Mouse CPM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Mouse CPM is greater than 95% as determined by SEC-HPLC. |
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