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Mouse FGF21 Protein, Ultra Low Endotoxin (FGF-MM121-UL) Summary
Product Description |
Recombinant Mouse FGF21 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Ala29-Ser210. |
Product Background |
Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse FGF21 Protein, Ultra Low Endotoxin (FGF-MM121-UL) Specifications/Details
Protein |
FGF21 |
Synonyms |
UNQ3115; PRO10196; FGF-21; FGF21 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ala29-Ser210 |
Molecular Weight |
The protein has a predicted MW of 21 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 41.50 nM as determined in SPR assay (Biacore T200). |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
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Mouse FGF21, His Tag immobilized on CM5 Chip can bind Human Beta Klotho, His Tag with an affinity constant of 41.50 nM as determined in SPR assay (Biacore T200). |
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Mouse FGF21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse FGF21 is greater than 95% as determined by SEC-HPLC. |
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