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Mouse HMGB1 Protein, Ultra Low Endotoxin (HMG-MM1B1-UL) Summary
Product Description |
Recombinant Mouse HMGB1 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Met1-Glu215. |
Product Background |
High-mobility group box 1 (HMGB1) is a ubiquitous nuclear protein that promotes inflammation when released extracellularly after cellular activation, stress, damage or death. HMGB1 operates as one of the most intriguing molecules in inflammatory disorders via recently elucidated signal and molecular transport mechanisms. Treatments based on antagonists specifically targeting extracellular HMGB1 have generated encouraging results in a wide number of experimental models of infectious and sterile inflammation. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse HMGB1 Protein, Ultra Low Endotoxin (HMG-MM1B1-UL) Specifications/Details
Protein |
HMGB1 |
Synonyms |
HMGB1; HMG1; HMG3; SBP-1; HMG-1 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Met1-Glu215 |
Molecular Weight |
The protein has a predicted MW of 25.71 kDa. Due to glycosylation, the protein migrates to 26-30 kDa based on Tris-Bis PAGE result. |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Mouse HMGB1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Mouse HMGB1 is greater than 95% as determined by SEC-HPLC. |
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