Mouse HPX Protein (HPX-MM101)
Mouse HPX Protein (HPX-MM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Ser24-Gln460
Catalog #HPX-MM101
Regular price $270.00
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Product Description
Recombinant Mouse HPX Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser24-Gln460.
Product Background
Hemopexin (HPX) serves as scavenger and transporter of toxic plasma heme to the liver. HPX is formed by two four-bladed beta-propeller domains, resembling two thick disks that lock together at a 90 degrees angle. The heme is bound between the two beta-propeller domains in a pocket formed by the interdomain linker peptide.HPX, acting not only as a heme carrier but also displaying transient heme-based ligand binding and (pseudo-)enzymatic properties, could be considered a 'chronosteric' heme-protein.
Product Category
Recombinant Protein
Target
HPX
Synonyms
Hemopexin;Hpx;Hpxn
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Ser24-Gln460
Molecular Weight
The protein has a predicted MW of 50 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Bis-Tris PAGE result.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 50mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM MES, 150mM NaCl (pH 6.0).
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse HPX on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse HPX is greater than 95% as determined by SEC-HPLC.

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