Name: Mouse HPX Protein (HPX-MM101)
Brand: KactusBio
SKU: HPX-MM101-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Measured by its ability to bind protoporphyrin IX (PPPIX). Recombinant Mouse Hemopexin binds > 20 uM PPPIX, resulting in a 50% decrease in the fluorescence signal of Mouse Hemopexin.
Amino Acid Range: Ser24-Gln460

Catalog #HPX-MM101

Size

100UG

500UG

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Mouse HPX Protein (HPX-MM101) Summary

Product Description	Recombinant Mouse HPX Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser24-Gln460.
Product Background	Hemopexin (HPX) serves as scavenger and transporter of toxic plasma heme to the liver. HPX is formed by two four-bladed beta-propeller domains, resembling two thick disks that lock together at a 90 degrees angle. The heme is bound between the two beta-propeller domains in a pocket formed by the interdomain linker peptide.HPX, acting not only as a heme carrier but also displaying transient heme-based ligand binding and (pseudo-)enzymatic properties, could be considered a 'chronosteric' heme-protein.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Mouse HPX Protein (HPX-MM101) Specifications/Details

Protein	HPX
Synonyms	Hemopexin;Hpx;Hpxn
Accession	Q91X72
Species	Mouse
Biotinylated	no
Amino Acid Range	Ser24-Gln460
Molecular Weight	The protein has a predicted MW of 50 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Bis-Tris PAGE result.
Activity	Measured by its ability to bind protoporphyrin IX (PPPIX). Recombinant Mouse Hemopexin binds > 20 uM PPPIX, resulting in a 50% decrease in the fluorescence signal of Mouse Hemopexin.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 50mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 50mM MES, 150mM NaCl (pH 6.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Mouse HPX on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse HPX is greater than 95% as determined by SEC-HPLC.