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Mouse ICAM-1/CD54 Protein, Ultra Low Endotoxin (ICM-MM101-UL) Summary
Product Description |
Recombinant Mouse ICAM-1/CD54 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln28-Asn485. |
Product Background |
Intercellular adhesion molecule-1 (ICAM-1; CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and in animal models of atherosclerosis. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Mouse ICAM-1/CD54 Protein, Ultra Low Endotoxin (ICM-MM101-UL) Specifications/Details
Protein |
ICAM-1/CD54 |
Synonyms |
ICAM-1; BB2; CD54 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Gln28-Asn485 |
Molecular Weight |
The protein has a predicted MW of 51.3 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Mouse ICAM-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Mouse ICAM-1 is greater than 95% as determined by SEC-HPLC. |
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