Mouse Kallikrein 5/KLK5 Protein (active form), Ultra Low Endotoxin (KLK-MM105-UL) Summary
Product Description |
Recombinant Mouse Kallikrein 5/KLK5 Protein (active form) is expressed from HEK293 with His tag at the C-terminus.It contains Gly30-Asn293. |
Product Background |
The inhibition of kallikrein 5 (KLK5) has been identified as a potential strategy for treatment of the genetic skin disorder Netherton syndrome, in which loss-of-function mutations in the SPINK5 gene lead to down-regulation of the endogenous inhibitor LEKTI-1 and profound skin-barrier defects with severe allergic manifestations. To aid in the development of a medicine for this target, an X-ray crystallographic system was developed to facilitate fragment-guided chemistry and knowledge-based drug-discovery approaches. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Mouse Kallikrein 5/KLK5 Protein (active form), Ultra Low Endotoxin (KLK-MM105-UL) Specifications/Details
Protein |
Kallikrein 5/KLK5 |
Synonyms |
Kallikrein c; Klnc; KLK5; KLKL2; KLK-L2; SCTE |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Gly30-Asn293 |
Molecular Weight |
The protein has a predicted MW of 29.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >5000 pmol/min/ug. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 20mM NaAc, 150mM NaCl, 8% trehalose (pH 5.0). |
Reconstitution |
Dissolve the lyophilized protein in 20mM NaAC (pH 5.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
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Mouse Kallikrein 5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse Kallikrein 5 is greater than 95% as determined by SEC-HPLC. |
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