Mouse Kallikrein 5/KLK5 Protein (active form), Ultra Low Endotoxin (KLK-MM105-UL)

SKU: KLK-MM105-UL-100UG

Mouse Kallikrein 5/KLK5 Protein (active form), Ultra Low Endotoxin (KLK-MM105-UL)

SKU: KLK-MM105-UL-100UG
Mouse Kallikrein 5/KLK5 Protein (active form), Ultra Low Endotoxin (KLK-MM105-UL)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >5000 pmol/min/ug.
  • Amino Acid Range: Gly30-Asn293
Catalog #KLK-MM105-UL
/
Size
image image

Order by 4pm ET for delivery on:

Mouse Kallikrein 5/KLK5 Protein (active form), Ultra Low Endotoxin (KLK-MM105-UL) Summary

Product Description

 Recombinant Mouse Kallikrein 5/KLK5 Protein (active form) is expressed from HEK293 with His tag at the C-terminus.It contains Gly30-Asn293.

Product Background

 The inhibition of kallikrein 5 (KLK5) has been identified as a potential strategy for treatment of the genetic skin disorder Netherton syndrome, in which loss-of-function mutations in the SPINK5 gene lead to down-regulation of the endogenous inhibitor LEKTI-1 and profound skin-barrier defects with severe allergic manifestations. To aid in the development of a medicine for this target, an X-ray crystallographic system was developed to facilitate fragment-guided chemistry and knowledge-based drug-discovery approaches.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Mouse Kallikrein 5/KLK5 Protein (active form), Ultra Low Endotoxin (KLK-MM105-UL) Specifications/Details

Protein
 Kallikrein 5/KLK5
Synonyms
 Kallikrein c; Klnc; KLK5; KLKL2; KLK-L2; SCTE
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Gly30-Asn293
Molecular Weight
 The protein has a predicted MW of 29.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
 Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >5000 pmol/min/ug.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in 20mM NaAc, 150mM NaCl, 8% trehalose (pH 5.0).
Reconstitution
 Dissolve the lyophilized protein in 20mM NaAC (pH 5.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse Kallikrein 5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse Kallikrein 5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse Kallikrein 5 is greater than 95% as determined by SEC-HPLC. The purity of Mouse Kallikrein 5 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recommended Products

Loading...
Recently viewed