SEC-HPLC chromatogram showing a main protein peak at approximately 7.426 minutes, with smaller secondary peaks at 9.486 and 10.754 minutes, indicating high purity with minor impurities or degradation products.
Mouse LRRN1 Protein (LNR-MM101)
SEC-HPLC chromatogram showing a main protein peak at approximately 7.426 minutes, with smaller secondary peaks at 9.486 and 10.754 minutes, indicating high purity with minor impurities or degradation products.
Mouse LRRN1 Protein (LNR-MM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Amino Acid Range: Ser26-Ala631
  • Other Available Proteins:
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Catalog #LNR-MM101
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Mouse LRRN1 Protein (LNR-MM101) Summary

Product Description

 Recombinant Mouse LRRN1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser26-Ala631.

Product Background

 Lrrn1 is required for the formation of MHB--loss of function leads to a loss of the morphological constriction and loss of Fgf8. Cells overexpressing Lrrn1 violate the boundary and result in a loss of cell restriction between midbrain and hindbrain compartments. Lrrn1 also regulates the glycosyltransferase Lunatic Fringe, a modulator of Notch signalling, maintaining its expression in midbrain cells which is instrumental in MHB boundary formation.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Mouse LRRN1 Protein (LNR-MM101) Specifications/Details

Protein
 LRRN1
Synonyms
 Leucine-rich repeat neuronal protein 1; Neuronal leucine-rich repeat protein 1; NLRR-1; NLRR1; FIGLER3; KIAA1497; LRN
Accession
Species
 Mouse
Biotinylated
 no
Amino Acid Range
 Ser26-Ala631
Molecular Weight
 The protein has a predicted MW of 69.28 kDa. Due to glycosylation, the protein migrates to 80-90 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Mouse LRRN1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse LRRN1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse LRRN1 is greater than 90% as determined by SEC-HPLC. The purity of Mouse LRRN1 is greater than 90% as determined by SEC-HPLC.
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