Mouse TNFSF15 Protein (FSF-MM215)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Mouse DR3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFSF15, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test).. The affinity constant of 4.56 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ala61-Leu252

Catalog: FSF-MM215

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Product Description	Recombinant Mouse TNFSF15 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala61-Leu252.
Product Background	TL1A and its functional receptor DR3 are members of the TNF/TNFR superfamilies of proteins.TL1A and DR3 are abundantly localized at inflamed intestinal areas of patients with IBD and mice with experimental ileitis or colitis and actively participate in the immunological pathways that underlie mucosal homeostasis and intestinal inflammation.Recently, an important role was demonstrated for TL1A/DR3 as potential mediators of intestinal fibrosis that is associated with the presence of gut inflammation.
Product Category	Recombinant Protein

Target	TNFSF15
Synonyms	TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A
Gene/Protein ID	Q5UBV8
Species	Mouse
Exact Sequence	Ala61-Leu252
Molecular Weight	The protein has a predicted MW of 48.8 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse DR3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFSF15, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test).. The affinity constant of 4.56 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Mouse TNFSF15, hFc Tag captured on CM5 Chip via Protein A can bind Mouse DR3, His Tag with an affinity constant of 4.56 nM as determined in SPR assay (Biacore T200).
	Immobilized Mouse DR3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFSF15, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test)..
	The purity of Mouse TNFSF15 is greater than 95% as determined by SEC-HPLC.
	Mouse TNFSF15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.