Product Description |
Recombinant Mouse TNFSF15 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala61-Leu252. |
Product Background |
TL1A and its functional receptor DR3 are members of the TNF/TNFR superfamilies of proteins.TL1A and DR3 are abundantly localized at inflamed intestinal areas of patients with IBD and mice with experimental ileitis or colitis and actively participate in the immunological pathways that underlie mucosal homeostasis and intestinal inflammation.Recently, an important role was demonstrated for TL1A/DR3 as potential mediators of intestinal fibrosis that is associated with the presence of gut inflammation. |
Product Category |
Recombinant Protein |
Target |
TNFSF15 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ala61-Leu252 |
Molecular Weight |
The protein has a predicted MW of 24.40 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse TNFSF15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.96 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
N-His-Avi |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Mouse DR3, His Tag immobilized on CM5 Chip can bind Mouse TNFSF15, His Tag with an affinity constant of 0.96 nM as determined in SPR assay (Biacore T200). |
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Immobilized Mouse TNFSF15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). |
|
|
Mouse TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Mouse TNFSF15 is greater than 95% as determined by SEC-HPLC. |
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