mRNA Cap 2'-O-Methyltransferase, GMP Grade (GMP-MEH-VE101)

  • Quality Statement:
    Manufactured to the GMP guidelines

    Antibiotic-free and animal-free production

    Suitable for manufacturing of mRNA therapeutics and vaccines
  • Express System: E.coli

Catalog: GMP-MEH-VE101

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Product Description mRNA Cap 2'-O-Methyltransferase, utilizing S-adenosylmethionine (SAM) as a methyl group donor, adds a methyl group at the 2'-O position of the first nucleotide adjacent to the Cap0 structure at the 5' end of RNA to obtain m7Gppp5'mN Cap1 structure. Cap1 structure as a signature of self RNA.and has been shown to modulate binding of innate immune sensors. For instance, the binding affinity of IFIT-1 for Cap1 is much weaker than for 5' triphosphate or Cap0 structure; Cap1 structure abrogates RIG-1 signaling and prevents detection by MDA5.
Expressed in an E.coli strain with the gene of mRNA Cap 2'-O-Methyltransferase.
Product Category GMP Enzyme
Application RNA Modification
Concentration 50 U/UL
Unit Definition One unit is defined as the amount of enzyme required to methylate 10 pmoles of 80 nt long capped RNA transcript in 1 hour at 37°C.
Express System E.coli
Quality Statement
Manufactured to the GMP guidelines

Antibiotic-free and animal-free production

Suitable for manufacturing of mRNA therapeutics and vaccines
QC Standard Activity: >=67.7 kU/ml
Purity: >=95%
Residual Endonuclease: Negative
Residual Exonuclease: Negative
Residual RNase: Negative
Residual Protease: Negative
Endotoxin: <=10 EU/ml
Residual Host Cell DNA: <=100 pg/MG
Residual Host Protein: <=50 ng/MG
Bioburden: <=1 CFU/10 ml
Form Liquid
Shipping Shipped with blue ice
Stability And Storage -20°C for 12 months. Avoid repeated freeze-thaw cycles.
Kactus mRNA capping system delivers 99%+ capping efficiency

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