mRNA Cap 2'-O-Methyltransferase, GMP grade (GMP-MEH-VE101)

  • Description: Adds a methyl group at the 2'-O position of the first nucleotide adjacent to the Cap0 structure at the 5' end of RNA to obtain m7Gppp5'mN Cap1 structure
  • Applications: RNA Modification
  • Quality: Manufactured accoridng to GMP guidelines
    Antibiotic-free and animal-free production
    Suitable for manufacturing of mRNA therapeutics and vaccines
  • Related Products: Vaccinia Capping Enzyme (#GMP-VCS-VE101)

Catalog: GMP-MEH-VE101

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Product Description mRNA Cap 2'-O-Methyltransferase, utilizing S-adenosylmethionine (SAM) as a methyl group donor, adds a methyl group at the 2'-O position of the first nucleotide adjacent to the Cap0 structure at the 5' end of RNA to obtain m7Gppp5'mN Cap1 structure. Cap1 structure as a signature of self RNA.and has been shown to modulate binding of innate immune sensors. For instance, the binding affinity of IFIT-1 for Cap1 is much weaker than for 5' triphosphate or Cap0 structure; Cap1 structure abrogates RIG-1 signaling and prevents detection by MDA5.
Application RNA Modification
Concentration 50U/µL
Unit Definition One unit is defined as the amount of enzyme required to methylate 10 pmoles of 80 nt long capped RNA transcript in 1 hour at 37°C.
Source Expressed in an E.coli strain with the gene of mRNA Cap 2'-O-Methyltransferase.
Quality Standards Activity (Capping modification and efficiency measurement): ≥ 50 kU/mL
Purity (SEC-HPLC): ≥ 95%
Residual Endonuclease: Negative
Residual Exonuclease: Negative
Residual DNase: Negative
Residual RNase: Negative
Residual Protease: Negative
Endotoxin: ≤ 10 EU/mL
Residual Host Cell DNA: ≤ 100 pg/mg
Residual Host Protein: ≤ 20 ng/mg
Residual Heavy Metal: ≤ 10 ppm
Bioburden: ≤ 1 CFU/10mL
Form Liquid
Shipping Shipped with blue ice
Stability And Storage -20±5°C for 12 months. Avoid repeated freeze-thaw cycles.

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