Vaccinia Capping Enzyme, GMP grade (GMP-VCS-VE101)

  • Description: Vaccinia Capping Enzyme adds the 7-methylguanosine cap structure (Cap0) structure to the 5' end of mRNA. Cap0 is a characteristic feature of RNA transcribed by RNA-polymerase II (i.e. mRNA) and is essential for efficient translation of mRNA in eukaryotic cells.
  • Applications: In vitro transcription, RNA modification, Labeling 5’ end of mRNA
  • Quality: GMP-Grade, Antibiotic-free and animal-free production, Suitable for manufacturing of mRNA therapeutics, Regulatory support documents available
  • Related Products: mRNA Cap 2'-O-Methyltransferase, GMP-MEH-VE101

Catalog: GMP-VCS-VE101


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Product Description The 7-methylguanosine cap structure(m7Gppp, Cap0) is a specific feature in eukaryotic mRNA, which is required for mRNA stability, splicing, nuclear export, effective translation, and mRNA decay. Vaccinia Capping Enzyme, a heterodimer of D1(844 aa) and D12(287 aa) subunits, adding the Cap0 structure to the 5' end of the RNA. The D1 subunit comprises an N-terminal RNA triphosphatase(TPase), a guanylyltransferase(GTase), and a C-terminal guanine-N7-methyltransferase(MTase). The D12 subunit binds and stimulates the MTase.
mRNA Cap 2'-O-Methyltransferase (Kactus #GMP-MEH-VE101) is required to generate Cap 1 structure that reduces cellular innate immune response when the RNA is used in vivo.
Application In vitro transcription, RNA modification, Labeling 5’ end of mRNA
Concentration 10U/µL
Unit Definition One unit is defined as the amount of enzyme required to incorporate 10 pmol of (α32P) GTP into an 80 nt transcript in 1 hour at 37°C.
Source E. coli
Quality Standards Activity (Capping modification and efficiency measurement): ≥10 kU/mL
Purity (SEC-HPLC): ≥95%
Residual Endonuclease: Negative
Residual Exonuclease: Negative
Residual DNase: Negative
Residual RNase: Negative
Residual Protease: Negative
Endotoxin: ≤10EU/mL
Residual Host Cell DNA: ≤100pg/mL
Residual Host Protein: ≤20ng/mg
Residual Heavy Metal: 10 ppm
Bioburden: ≤1 CFU/10mL
Form Liquid
Shipping Shipped with blue ice
Stability And Storage -20±5°C for 24 months. Avoid repeated freeze-thaw cycles.
Figure 1. Analysis of mRNA capping efficiency using LC-MS.

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