Vaccinia Capping Enzyme, GMP Grade (GMP-VCS-VE101)

  • Quality Statement:
    Manufactured to the GMP guidelines

    Antibiotic-free and animal-free production

    Suitable for manufacturing of mRNA therapeutics and vaccines
  • Express System: E.coli

Catalog: GMP-VCS-VE101

$2,125.00
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Product Description The 7-methylguanosine cap structure(m7Gppp, Cap0) is a specific feature in eukaryotic mRNA, which is required for mRNA stability, splicing, nuclear export, effective translation, and mRNA decay. Vaccinia Capping Enzyme, a heterodimer of D1(844 aa) and D12(287 aa) subunits, adding the Cap0 structure to the 5' end of the RNA. The D1 subunit comprises an N-terminal RNA triphosphatase(TPase), a guanylyltransferase(GTase), and a C-terminal guanine-N7-methyltransferase(MTase). The D12 subunit binds and stimulates the MTase.
mRNA Cap 2'-O-Methyltransferase (Kactus #GMP-MEH-VE101) is required to generate Cap 1 structure that reduces cellular innate immune response when the RNA is used in vivo.
Expressed in an E.coli strain carries the genes of the Vaccinia virus Capping Enzyme.
Product Category GMP Enzyme
Application RNA Modification
In Vitro Synthesis(IVT)
Labeling 5' end of mRNA
Concentration 10 U/UL
Unit Definition One unit is defined as the amount of enzyme required to incorporate 10 pmol of (α32P) GTP into an 80 nt transcript in 1 hour at 37°C.
Express System E.coli
Quality Statement
Manufactured to the GMP guidelines

Antibiotic-free and animal-free production

Suitable for manufacturing of mRNA therapeutics and vaccines
QC Standard Activity: >=12.7 kU/ml
Purity: >=95%
Residual Endonuclease: Negative
Residual Exonuclease: Negative
Residual RNase: Negative
Residual Protease: Negative
Endotoxin: <=10 EU/ml
Residual Host Cell DNA: <=100 pg/MG
Residual Host Protein: <=50 ng/MG
Bioburden: <=1 CFU/10 ml
Form Liquid
Shipping Shipped with blue ice
Stability And Storage -20°C for 12 months. Avoid repeated freeze-thaw cycles.
Kactus mRNA capping system delivers 99%+ capping efficiency

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