Regular price
$381.00
Order by 4pm ET for delivery on:
Non-biotinylated-Cynomolgus-IL-17A/CTLA-8-Protein,-Ultra Low Endotoxin (ILA-CM417-UL) Summary
Product Description |
Recombinant Cynomolgus IL-17A/CTLA-8 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-Ala155. |
Product Background |
Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Non-biotinylated-Cynomolgus-IL-17A/CTLA-8-Protein,-Ultra Low Endotoxin (ILA-CM417-UL) Specifications/Details
Protein |
IL-17A/CTLA-8 |
Synonyms |
CTLA-8; IL17; IL17A; IL-17CTLA-8; interleukin 17A |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Gly24-Ala155 |
Molecular Weight |
The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 26-32 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus IL-17A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Cynomolgus IL-17A, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA. |
|
|
Immobilized Cynomolgus IL-17A, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. |
|
|
Cynomolgus IL-17A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recommended Products
Loading...
Recently viewed
