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Non-biotinylated Human ANGPTL2/Angiopoietin-like 2 Protein, Ultra Low Endotoxin (ANG-HM4L2-UL) Summary
Product Description |
Recombinant Human ANGPTL2/Angiopoietin-like 2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr260-His493. |
Product Background |
Angiopoietin-like 2 (ANGPTL2) is a proinflammatory protein belonging to the angiopoietin-like family. ANGPTL2 is secreted and detected in the systemic circulation. Different observational clinical studies reported that circulating levels of ANGPTL2 increase significantly in various chronic inflammatory diseases and showed associations between ANGPTL2 levels and diagnosis and/or prognosis of cardiovascular diseases, diabetes, chronic kidney disease, and various types of cancers. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Non-biotinylated Human ANGPTL2/Angiopoietin-like 2 Protein, Ultra Low Endotoxin (ANG-HM4L2-UL) Specifications/Details
Protein |
ANGPTL2/Angiopoietin-like 2 |
Synonyms |
ARP2; HARP; angiopoietin-like 2; ANGPTL2; ANGRP2; MGC8889 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Thr260-His493 |
Molecular Weight |
The protein has a predicted MW of 30.1 kDa. Due to glycosylation, the protein migrates to 31-35 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His-Avi |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human ANGPTL2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human ANGPTL2 is greater than 95% as determined by SEC-HPLC. |
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