Pyrophosphatase, Inorganic (PYR-EE201)

  • Applications: In vitro transcription (IVT)
    Optimal PH 7.0
    Cofactor Mg2+ is required for enzymatic activity
  • Express System: E.coli

Catalog: PYR-EE201

$28.00
Size:

Order now get it on

Product Description Pyrophosphatase hydrolyzes inorganic pyrophosphate generated during in vitro transcription reactions. By removing unwanted pyrophosphate, the equilibrium is shifted to the product side increasing RNA yield.
A PH 7.0 is ideal for optimal performance and the cofactor Mg2+ is required for enzymatic activity.
Expressed in an E.coli strain with the pyrophosphatase gene from yeast.

Product Category

Enzyme
Application In vitro transcription (IVT)
Optimal PH 7.0
Cofactor Mg2+ is required for enzymatic activity
Concentration 0.1U/UL
Unit Definition One unit of the enzyme hydrolysis 1µmol of inorganic pyrophosphate in 1 min at 25 °C. Enzyme activity is assayed in 100mM Tris-HCl (pH 7.2), 2mM MgCl2 and 2mM inorganic pyrophosphate (PPi).
Molecular Weight 63KD

Express System

E.coli
Form Liquid
Shipping Shipped with blue ice.
Stability And Storage -20°C for 12 months. Avoid repeated freeze-thaw cycles.

You may also be interested in