Product Description |
Pyrophosphatase hydrolyzes inorganic pyrophosphate generated during in vitro transcription reactions. By removing unwanted pyrophosphate, the equilibrium is shifted to the product side increasing RNA yield. A PH 7.0 is ideal for optimal performance and the cofactor Mg2+ is required for enzymatic activity. Expressed in an E.coli strain with the pyrophosphatase gene from yeast. |
Product Category |
Enzyme |
Applications |
In vitro transcription (IVT) Optimal PH 7.0 Cofactor Mg2+ is required for enzymatic activity |
Concentration |
0.1U/UL |
Unit Definition |
One unit of the enzyme hydrolysis 1µmol of inorganic pyrophosphate in 1 min at 25 °C. Enzyme activity is assayed in 100mM Tris-HCl (pH 7.2), 2mM MgCl2 and 2mM inorganic pyrophosphate (PPi). |
Biotinylated |
no |
Molecular Weight |
63KD |
Express System |
E.coli |
Form |
Liquid |
Shipping |
Shipped with blue ice. |
Stability And Storage |
-20°C for 12 months. Avoid repeated freeze-thaw cycles. |
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