Rat TNFSF15 Protein (FSF-RM215)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Rat TNFSF15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Thr61-Ile252

Catalog: FSF-RM215

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Product Description	Recombinant Rat TNFSF15 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Thr61-Ile252.
Product Background	TL1A and its functional receptor DR3 are members of the TNF/TNFR superfamilies of proteins.TL1A and DR3 are abundantly localized at inflamed intestinal areas of patients with IBD and mice with experimental ileitis or colitis and actively participate in the immunological pathways that underlie mucosal homeostasis and intestinal inflammation.Recently, an important role was demonstrated for TL1A/DR3 as potential mediators of intestinal fibrosis that is associated with the presence of gut inflammation.
Product Category	Recombinant Protein

Target	TNFSF15
Accession	Q8K3Y7
Species	Rat
Amino Acid Range	Thr61-Ile252
Molecular Weight	The protein has a predicted MW of 22.76 kDa. Due to glycosylation, the protein migrates to 32-40 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Rat TNFSF15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Rat TNFSF15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA.
	The purity of Rat TNFSF15 is greater than 95% as determined by SEC-HPLC.
	Rat TNFSF15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.