Rhesus macaque IL-18 R1/CD218a Protein (IL8-RM2R1)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Rhesus macaque IL-18 R1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 0.42ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Cys22-Gly329
Catalog #IL8-RM2R1
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Product Description
 Recombinant Rhesus macaque IL-18 R1/CD218a Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Cys22-Gly329.
Product Background
 Interleukin-18 (IL-18) is a member of the interleukin-1 family of cytokines produced constitutively by different cell types and by adipose tissue. Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.
Product Category
 Recombinant Protein
Target
 IL-18 R1/CD218a
Accession
Species
 Rhesus macaque
Biotinylated
 no
Amino Acid Range
 Cys22-Gly329
Molecular Weight
 The protein has a predicted MW of 61.96 kDa. Due to glycosylation, the protein migrates to 75-95 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Rhesus macaque IL-18 R1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 0.42ug/ml determined by ELISA. See testing image for detail.
Product Tag
 C-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Rhesus macaque IL-18 R1, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 0.42ug/ml determined by ELISA.
Rhesus macaque IL-18 R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Rhesus macaque IL-18 R1 is greater than 95% as determined by SEC-HPLC.

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