RSV (A) Pre-fusion glycoprotein F0 Protein (RSV-VM1PR)
|Recombinant RSV (A) Pre-fusion glycoprotein F0 Protein is expressed from HEK293 with His tag at the C-terminus.
|Respiratory syncytial virus (RSV) is a highly contagious childhood pathogen of the respiratory tract and is divided into two antigenic subtypes, A and B, based on the reactivity of the F and G surface proteins to monoclonal antibodies. Surface protein F (fusion protein) is responsible for fusion of viral and host cell membranes, as well as syncytium formation between viral particles. Its sequence is highly conserved between strains. F protein exists in multiple conformational forms. In the prefusion state (PreF), the protein exists in a trimeric form and contains the major antigenic site Ø which serves as a primary target of neutralizing antibodies in the body.
|Pre-fusion glycoprotein F0
|The protein has a predicted MW of 56.28 kDa. Due to glycosylation, the protein migrates to 57-70 kDa based on Tris-Bis PAGE result.
|Immobilized RSV (A) Pre-fusion glycoprotein F0, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (D25) Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. See testing image for detail.
|> 95% as determined by Tris-Bis PAGE
|Less than 1EU per ug by the LAL method.
|Shipped at ambient temperature.
|Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
|Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
|Stability And Storage
|Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.