- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE
- Activity: Immobilized HRSV (A, strain A2) Pre-fusion glycoprotein F0, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (D25) Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Description |
Recombinant HRSV (A, strain A2) Pre-fusion glycoprotein F0 Protein is expressed from HEK293 with His tag at the C-terminus. |
Product Background |
Respiratory syncytial virus (RSV) is a highly contagious childhood pathogen of the respiratory tract and is divided into two antigenic subtypes, A and B, based on the reactivity of the F and G surface proteins to monoclonal antibodies. Surface protein F (fusion protein) is responsible for fusion of viral and host cell membranes, as well as syncytium formation between viral particles. Its sequence is highly conserved between strains. F protein exists in multiple conformational forms. In the prefusion state (PreF), the protein exists in a trimeric form and contains the major antigenic site Ø which serves as a primary target of neutralizing antibodies in the body. |
Product Category |
Recombinant Protein |
Target |
Pre-fusion glycoprotein F0 |
Species |
HRSV (A, strain A2) |
Biotinylated |
no |
Molecular Weight |
The protein has a predicted MW of 56.28 kDa. Due to glycosylation, the protein migrates to 57-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized HRSV (A, strain A2) Pre-fusion glycoprotein F0, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Fusion glycoprotein F0 (D25) Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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