SARS-COV-2 Spike S1 (D614G) Protein (COV-VM4SG)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized SARS-CoV-2 S1 (D614G), His Tag at 2μg/ml (100μl/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 42ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln14-Arg683 (D614G)

Catalog: COV-VM4SG

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Product Description Recombinant SARS-COV-2 Spike S1 (D614G) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Gln14-Arg683 (D614G).
Product Category Recombinant Protein
Target Spike S1 (D614G)
Synonyms S1 protein; Spike protein S1; Spike, S1 protein; S glycoprotein Subunit1
Gene/Protein ID QHD43416.1
Species SARS-COV-2
Exact Sequence Gln14-Arg683 (D614G)
Molecular Weight The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.
Activity Immobilized SARS-CoV-2 S1 (D614G), His Tag at 2μg/ml (100μl/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 42ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized SARS-CoV-2 S1 (D614G), His Tag at 2μg/ml (100μl/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 42ng/ml determined by ELISA.
The purity of SARS-COV-2 Spike S1 (D614G) is greater than 95% as determined by SEC-HPLC.
SARS-COV-2 Spike S1 (D614G) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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