SARS-COV-2 Spike S1 (D614G) Protein (COV-VM4SG)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized SARS-CoV-2 S1 (D614G) , His Tag at 2ug/ml (100ul/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln14-Arg683(D614G)

Catalog: COV-VM4SG

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Product Description Recombinant SARS-COV-2 Spike S1 (D614G) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln14-Arg683(D614G).

Product Background

The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.

Product Category

Recombinant Protein

Target

Spike S1 (D614G)
Synonyms S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1

Gene/Protein ID

QHD43416.1

Species

SARS-COV-2

Exact Sequence

Gln14-Arg683(D614G)
Molecular Weight The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.

Activity

Immobilized SARS-CoV-2 S1 (D614G) , His Tag at 2ug/ml (100ul/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized SARS-CoV-2 S1 (D614G) , His Tag at 2ug/ml (100ul/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA.
The purity of SARS-COV-2 Spike S1 (D614G) is greater than 95% as determined by SEC-HPLC.
SARS-COV-2 Spike S1 (D614G) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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