SARS-COV-2 Spike S1 (D614G) Protein (COV-VM4SG)
Catalog: COV-VM4SG
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| Product Description | Recombinant SARS-COV-2 Spike S1 (D614G) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gln14-Arg683(D614G). |
| Product Category | Recombinant Protein |
| Target | Spike S1 (D614G) |
| Synonyms | S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1 |
| Gene/Protein ID | QHD43416.1 |
| Species | SARS-COV-2 |
| Exact Sequence | Gln14-Arg683(D614G) |
| Molecular Weight | The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized SARS-CoV-2 S1 (D614G) , His Tag at 2μg/ml (100μl/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 42ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized SARS-CoV-2 S1 (D614G) , His Tag at 2μg/ml (100μl/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 42ng/ml determined by ELISA. |
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The purity of SARS-COV-2 Spike S1 (D614G) is greater than 95% as determined by SEC-HPLC. |
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SARS-COV-2 Spike S1 (D614G) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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