T4 RNA Ligase I (TRL-BE101)

  • Description: Catalyzes the 5' phosphate group of the donor and the 3' hydroxyl group of the ligand to form a 3' → 5' phosphodiester bond
  • Applications: RNA circularization
    Ligation of RNA and RNA/DNA
    Intramolecular and intermolecular connections
    between DNA and RNA
    Connection of 5' end adapters in miRNA library construction
    RNA 3' end labeling

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Product Description T4 RNA Ligase I is an ATP-dependent single-stranded nucleic acid molecule (DNA or RNA) ligase, which catalyzes the 5' phosphate group of the donor and the 3' hydroxyl group of the ligand to form a 3' → 5' phosphodiester bond and is accompanied by the hydrolysis of ATP to AMP and PPi. The substrates of this enzyme are ssRNA, ssDNA, and dinucleoside pyrophosphate.
Application RNA circularization
Ligation of RNA and RNA/DNA
Intramolecular and intermolecular connections
between DNA and RNA
Connection of 5' end adapters in miRNA library construction
RNA 3' end labeling
Concentration 30U/µL
Unit Definition One unit is defined as the amount of enzyme required to convert 1 nmol of 5’-[32P] rA16 into phosphate-insoluble matter at 37°C.
Source E. coli strains carrying the T4 RNA ligase I gene
Form Liquid
Shipping Shipped with dry ice.
Stability And Storage Store at -20°C ± 5°C for 24 months. Avoid repeated freeze/thaw cycles.
Example standard curve for standard and rapid protocol.

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