T4 RNA Ligase I (TRL-BE101)
  • Description: Catalyzes the 5' phosphate group of the donor and the 3' hydroxyl group of the ligand to form a 3' → 5' phosphodiester bond
  • Applications: RNA circularization
    Ligation of RNA and RNA/DNA
    Intramolecular and intermolecular connections
    between DNA and RNA
    Connection of 5' end adapters in miRNA library construction
    RNA 3' end labeling
  • Related Products:

    T4 RNA Ligase II

Catalog #TRL-BE101
Regular price $163.00
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Product Description
T4 RNA Ligase I is an ATP-dependent single-stranded nucleic acid molecule (DNA or RNA) ligase, which catalyzes the 5' phosphate group of the donor and the 3' hydroxyl group of the ligand to form a 3' → 5' phosphodiester bond and is accompanied by the hydrolysis of ATP to AMP and PPi. The substrates of this enzyme are ssRNA, ssDNA, and dinucleoside pyrophosphate.
Product Category
Enzyme
Synonyms
ssRNA Ligase; T4 RNA Ligase 1; Rnl1; T4Rnl1; T4 rnl1;
Applications
RNA circularization
Ligation of RNA and RNA/DNA
Intramolecular and intermolecular connections
between DNA and RNA
Connection of 5' end adapters in miRNA library construction
RNA 3' end labeling
Concentration
30U/µL
Unit Definition
One unit is defined as the amount of enzyme required to convert 1 nmol of 5’-[32P] rA16 into phosphate-insoluble matter at 37°C.
Source
E. coli strains carrying the T4 RNA ligase I gene
Biotinylated
no
Form
Liquid
Shipping
Shipped with dry ice.
Stability And Storage
Store at -20°C ± 5°C for 24 months. Avoid repeated freeze/thaw cycles.
Ligation of a 15mer RNA substrate using T4 RNA ligase I, followed by Urea page gel analysis.

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