Product Description |
T4 RNA Ligase I is an ATP-dependent single-stranded nucleic acid molecule (DNA or RNA) ligase, which catalyzes the 5' phosphate group of the donor and the 3' hydroxyl group of the ligand to form a 3' → 5' phosphodiester bond and is accompanied by the hydrolysis of ATP to AMP and PPi. The substrates of this enzyme are ssRNA, ssDNA, and dinucleoside pyrophosphate. |
Product Category |
Enzyme |
Synonyms |
ssRNA Ligase; T4 RNA Ligase 1; Rnl1; T4Rnl1; T4 rnl1; |
Applications |
RNA circularization Ligation of RNA and RNA/DNA Intramolecular and intermolecular connections between DNA and RNA Connection of 5' end adapters in miRNA library construction RNA 3' end labeling |
Concentration |
30U/µL |
Unit Definition |
One unit is defined as the amount of enzyme required to convert 1 nmol of 5’-[32P] rA16 into phosphate-insoluble matter at 37°C. |
Source |
E. coli strains carrying the T4 RNA ligase I gene |
Biotinylated |
no |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Stability And Storage |
Store at -20°C ± 5°C for 24 months. Avoid repeated freeze/thaw cycles. |
|
|
Ligation of a 15mer RNA substrate using T4 RNA ligase I, followed by Urea page gel analysis. |
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