T4 RNA Ligase I (TRL-BE101)

Description: Catalyzes the 5' phosphate group of the donor and the 3' hydroxyl group of the ligand to form a 3' → 5' phosphodiester bond
Applications: RNA circularization
Ligation of RNA and RNA/DNA
Intramolecular and intermolecular connections
between DNA and RNA
Connection of 5' end adapters in miRNA library construction
RNA 3' end labeling
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T4 RNA Ligase II

Catalog: TRL-BE101

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Product Description	T4 RNA Ligase I is an ATP-dependent single-stranded nucleic acid molecule (DNA or RNA) ligase, which catalyzes the 5' phosphate group of the donor and the 3' hydroxyl group of the ligand to form a 3' → 5' phosphodiester bond and is accompanied by the hydrolysis of ATP to AMP and PPi. The substrates of this enzyme are ssRNA, ssDNA, and dinucleoside pyrophosphate.
Product Category	Enzyme

Synonyms	ssRNA Ligase; T4 RNA Ligase 1; Rnl1; T4Rnl1; T4 rnl1;
Application	RNA circularization Ligation of RNA and RNA/DNA Intramolecular and intermolecular connections between DNA and RNA Connection of 5' end adapters in miRNA library construction RNA 3' end labeling
Concentration	30U/µL
Unit Definition	One unit is defined as the amount of enzyme required to convert 1 nmol of 5’-[32P] rA16 into phosphate-insoluble matter at 37°C.
Source	E. coli strains carrying the T4 RNA ligase I gene

Form	Liquid
Shipping	Shipped with dry ice.
Stability And Storage	Store at -20°C ± 5°C for 24 months. Avoid repeated freeze/thaw cycles.

	Ligation of a 15mer RNA substrate using T4 RNA ligase I, followed by Urea page gel analysis.
	The purity of T4 ligase I is greater than 95% as determined by Bis-Tris PAGE.