• Description: Adds a methyl group at the 2'-O position of the first nucleotide adjacent to the Cap0 structure at the 5' end of RNA to obtain m7Gppp5'mN Cap1 structure
  • Applications: RNA Modification
  • Quality: FDA Drug Master Files #038029
    Manufactured according to GMP guidelines
    Antibiotic-free and animal-free production
    Suitable for manufacturing of mRNA therapeutics and vaccines
Catalog #GMP-MEH-VE101
Price
Regular price $1,169.30
Regular price Sale price $1,169.30
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mRNA Cap 2'-O-Methyltransferase, GMP-Grade (GMP-MEH-VE101), DMF #038029 Summary

Product Description

 mRNA Cap 2'-O-Methyltransferase, utilizing S-adenosylmethionine (SAM) as a methyl group donor, adds a methyl group at the 2'-O position of the first nucleotide adjacent to the Cap0 structure at the 5' end of RNA to obtain m7Gppp5'mN Cap1 structure. Cap1 structure is a signature of self RNA.and has been shown to modulate binding of innate immune sensors. For instance, the binding affinity of IFIT-1 for Cap1 is much weaker than for 5' triphosphate or Cap0 structure; Cap1 structure abrogates RIG-1 signaling and prevents detection by MDA5.

NOTE: Vaccinia Capping Enzyme #GMP-VCS-VE101) is required to generate Cap0 structure.

This product has been filed with the FDA Drug Master Files (DMF) and is assigned DMF #038029.

mRNA Cap 2'-O-Methyltransferase, GMP-Grade (GMP-MEH-VE101), DMF #038029 Specifications/Details

Applications

 RNA Modification

Concentration

 50U/µL

Unit Definition

 One unit is defined as the amount of enzyme required to methylate 10 pmoles of 80 nt long capped RNA transcript in 1 hour at 37°C.

Source

 Expressed in an E.coli strain with the gene of mRNA Cap 2'-O-Methyltransferase.
Biotinylated
 no

Quality Standards

 Activity (Capping modification and efficiency measurement): ≥ 67.7 kU/mL
Purity (SEC-HPLC): ≥ 95%
Residual DNase: Negative
Residual RNase: Negative
Residual Protease: Negative
Endotoxin: ≤ 5 EU/mL
Residual Host Cell DNA: ≤ 100 pg/mg
Residual Host Protein: ≤ 20 ng/mg
Residual Heavy Metal: ≤ 10 ppm
Residual Nickel Salt: ≤ 10 ppm
Bioburden: ≤ 1 CFU/10mL

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with blue ice
Stability And Storage
 -20±5°C for 24 months. Avoid repeated freeze-thaw cycles.

Validation Images

Figure 1. Analysis of mRNA capping efficiency using LC-MS. Figure 1. Analysis of mRNA capping efficiency using LC-MS.
Other Available Proteins:
Finding available proteins...
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Vaccinia Capping Enzyme (#GMP-VCS-VE101)

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