Human VEGF165 Protein (VEG-HM065)
Human VEGF165 Protein (VEG-HM065)
Human VEGF165 Protein (VEG-HM065)
  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human VEGF165 at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGFR2, mFc Tag with the EC50 of 42.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala27-Arg191
Catalog #VEG-HM065
Regular price $285.00
/
Size

Order now, ships by:

Product Description

Recombinant Human VEGF165 Protein is expressed from HEK293 without tag.It contains Ala27-Arg191.

Product Background

Human papillomavirus (HPV) infection is an established risk factor for cervical carcinogenesis. VEGF165 was significantly higher, whereas VEGFC and VEGFD were significantly lower in malignant cervical carcinoma tissues as compared to normal cervix tissues. Expression levels of VEGF121 and VEGFC were significantly associated with type of tumor growth while VEGF165 was significantly associated with lymph node metastasis.

Product Category

Recombinant Protein

Protein

VEGF165

Synonyms

VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Ala27-Arg191

Molecular Weight

The protein has a predicted MW of 19.2 kDa. Due to glycosylation, the protein migrates to 20-30 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human VEGF165 at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGFR2, mFc Tag with the EC50 of 42.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

No Tag

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 0.05EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human VEGF165 at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGFR2, mFc Tag with the EC50 of 42.3ng/ml determined by ELISA. Immobilized Human VEGF165 at 1ug/ml (100ul/well) on the plate. Dose response curve for Human VEGFR2, mFc Tag with the EC50 of 42.3ng/ml determined by ELISA.
Immobilized Human VEGF165 at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 46.4ng/ml determined by ELISA (QC Test). Immobilized Human VEGF165 at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGFR1, His Tag with the EC50 of 46.4ng/ml determined by ELISA (QC Test).
Measured by a reporter gene assay using HEK293T-KDR-NFAT Cell line. The ED50-for this effect is < 10 ng/mL (QC Test). Measured by a reporter gene assay using HEK293T-KDR-NFAT Cell line. The ED50-for this effect is < 10 ng/mL (QC Test).
Human VEGF165 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human VEGF165 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed