Name: Mouse DR3/TNFRSF25 Protein (DR3-MM101)
Brand: KactusBio
SKU: DR3-MM101-100UG
Price: 315.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Activity: Immobilized Mouse DR3, His Tag at 1ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 21.4 ng/ml determined by ELISA (QC Test). The affinity constant of 4.56 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln31-Met196

Catalog #DR3-MM101

Size

100UG

500UG

Order now, ships by:

Product Description	Recombinant Mouse DR3/TNFRSF25 Protein is expressed from HEK293 with His tag at the C-Terminus.; It contains Gln31-Met196.
Product Background	Death Receptor 3 (DR3), also known as TNFRSF25, TRAMP, LARD, or WSL-1, is a death-domain-containing TNF-family receptor that, like its closest paralog TNFR1, binds the adaptor molecule TRADD through its cytoplasmic death domain. TRADD recruitment endows DR3 with dual-signaling capability to activate NF−κB and MAP-kinase signaling or alternatively trigger caspase activation and programmed cell death.
Product Category	Recombinant Protein

Protein	DR3/TNFRSF25
Synonyms	APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; TNFRSF25; LARD; TR3; TRAMP; WSL-LR
Accession	B1AWN9
Species	Mouse
Biotinylated	no
Amino Acid Range	Gln31-Met196
Molecular Weight	The protein has a predicted MW of 18.79 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse DR3, His Tag at 1ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 21.4 ng/ml determined by ELISA (QC Test). The affinity constant of 4.56 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Mouse TNFSF15, hFc Tag captured on CM5 Chip via Protein A can bind Mouse DR3, His Tag with an affinity constant of 4.56 nM as determined in SPR assay (Biacore T200).
	Immobilized Mouse DR3, His Tag at 1ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Mouse TNFSF15, His Tag with the EC50 of 21.4 ng/ml determined by ELISA (QC Test).
	Mouse DR3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse DR3 is greater than 95% as determined by SEC-HPLC.