Name: Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc (TCC-HM101)
Brand: KactusBio
SKU: TCC-HM101-100UG
Price: 1210.0 USD
Availability: InStock

Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc (TCC-HM101)

Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind OKT3, mFc Tag with an affinity constant of 0.20 nM as determined in SPR assay (Biacore T200).

Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc captured on CM5 Chip via Streptavidin can bind Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with an affinity constant of 0.78 nM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 18.3ng/ml determined by ELISA.

Immobilized Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag with the EC50 of 0.1ug/ml determined by ELISA.

Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind OKT3, mFc Tag with an affinity constant of 0.89 nM as determined in BLI assay (Gator® Prime).

Loaded Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with an affinity constant of 2.37 nM as determined in BLI assay (Gator® Prime).

Express System: HEK293
Product Tag: C-His
Activity: Immobilized Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 18.3ng/ml determined by ELISA. The affinity constant of 0.20 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: NY-ESO-1 specific TCR (1G4c113), CD3ε&CD3γ, CD3ε&CD3δ, and CD3ζ dimers

Catalog #TCC-HM101

Size

100UG

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Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc (TCC-HM101) Summary

Product Description	Recombinant Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc is expressed from HEK293. Both the biotin and the His tag are located on the C-terminus of the NY-ESO-1 TCR.It contains NY-ESO-1 specific TCR (1G4c113), CD3ε&CD3γ, CD3ε&CD3δ, and CD3ζ dimers.
Product Background	The 1G4c113 TCR is an engineered, affinity-matured variant of the 1G4 T-cell receptor that specifically recognizes the peptide fragment of NY-ESO-1 (SLLMWITQC) presented on the HLA-A*02:01. Cluster of Differentiation 3 (CD3) is a multimeric protein complex and T cell co-receptor that plays an essential role in adaptive immune responses. These CD3 dimers bind non-covalently to the TCR to form the TCR&CD3 complex on the cell surface.
Product Category	Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / Nanodisc

Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc (TCC-HM101) Specifications/Details

Protein	NY-ESO-1 TCR&CD3
Synonyms	NY-ESO-1 TCR&CD3
Species	Human
Biotinylated	yes
Amino Acid Range	NY-ESO-1 specific TCR (1G4c113), CD3ε&CD3γ, CD3ε&CD3δ, and CD3ζ dimers
Molecular Weight	The protein has a predicted MW of 194.8 kDa.
Activity	Immobilized Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 18.3ng/ml determined by ELISA. The affinity constant of 0.20 nM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Expression System	HEK293
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry.
Stability And Storage	Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind OKT3, mFc Tag with an affinity constant of 0.20 nM as determined in SPR assay (Biacore T200).
	Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc captured on CM5 Chip via Streptavidin can bind Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with an affinity constant of 0.78 nM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 18.3ng/ml determined by ELISA.
	Immobilized Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag with the EC50 of 0.1ug/ml determined by ELISA.
	Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind OKT3, mFc Tag with an affinity constant of 0.89 nM as determined in BLI assay (Gator® Prime).
	Loaded Biotinylated Human NY-ESO-1 TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with an affinity constant of 2.37 nM as determined in BLI assay (Gator® Prime).