Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc (TCC-HM102)

SKU: TCC-HM102-100UG

Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc (TCC-HM102)

SKU: TCC-HM102-100UG
Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc (TCC-HM102)
Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc (TCC-HM102)
Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc (TCC-HM102)
Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc (TCC-HM102)
  • Express System: HEK293
  • Product Tag: C-His
  • Activity: Immobilized Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 17.5ng/ml determined by ELISA. The affinity constant of 0.21 nM as determined in SPR assay (Biacore T200).
  • Recomended Products: Biotinylated Nanodisc Control (CON-HM1NB)
  • Amino Acid Range: KRAS G12D specific TCR (JD1a41b1), CD3ε&CD3γ, CD3ε&CD3δ, and CD3ζ dimers
Catalog #TCC-HM102
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Size
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Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc (TCC-HM102) Summary

Product Description

 Recombinant Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc is expressed from HEK293. Both the biotin and the His tag are located on the C-terminus of the KRAS G12D TCR.It contains KRAS G12D specific TCR (JD1a41b1), CD3ε&CD3γ, CD3ε&CD3δ, and CD3ζ dimers.

Product Background

 The JD1a41b1 TCR is an engineered, high-affinity T-cell receptor that strictly recognizes the peptide fragment of KRAS G12D (VVVGADGVGK) presented on the HLA-A*11:01 only. Cluster of Differentiation 3 (CD3) is a multimeric protein complex and T cell co-receptor that plays an essential role in adaptive immune responses. These CD3 dimers bind non-covalently to the TCR to form the TCR&CD3 complex on the cell surface.

Product Category

 Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / Nanodisc

Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc (TCC-HM102) Specifications/Details

Protein
 KRAS G12D TCR&CD3
Synonyms
 KRAS G12D TCR&CD3
Species
 Human
Biotinylated
 yes
Amino Acid Range
 KRAS G12D specific TCR (JD1a41b1), CD3ε&CD3γ, CD3ε&CD3δ, and CD3ζ dimers
Molecular Weight
 The protein has a predicted MW of 196.0 kDa.
Activity
 Immobilized Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 17.5ng/ml determined by ELISA. The affinity constant of 0.21 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His

Fluorophore

 Biotin-labeled
Expression System
 HEK293
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). Notice: Not recommended for flow cytometry.
Stability And Storage
 Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind OKT3, mFc Tag with an affinity constant of 0.21 nM as determined in SPR assay (Biacore T200). Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind OKT3, mFc Tag with an affinity constant of 0.21 nM as determined in SPR assay (Biacore T200).
Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with an affinity constant of 0.47 nM as determined in SPR assay (Biacore T200). Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with an affinity constant of 0.47 nM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 17.5ng/ml determined by ELISA. Immobilized Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 17.5ng/ml determined by ELISA.
Immobilized Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag with the EC50 of 0.53ug/ml determined by ELISA. Immobilized Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag with the EC50 of 0.53ug/ml determined by ELISA.
Loaded Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind OKT3, mFc Tag with an affinity constant of 0.90 nM as determined in BLI assay . Loaded Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind OKT3, mFc Tag with an affinity constant of 0.90 nM as determined in BLI assay .
Loaded Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with an affinity constant of 2.60 nM as determined in BLI assay . Loaded Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with an affinity constant of 2.60 nM as determined in BLI assay .
Other Available Proteins:
Finding available proteins...
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