Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc (TCC-HM102) Summary
Product Description |
Recombinant Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc is expressed from HEK293. Both the biotin and the His tag are located on the C-terminus of the KRAS G12D TCR.It contains KRAS G12D specific TCR (JD1a41b1), CD3ε&CD3γ, CD3ε&CD3δ, and CD3ζ dimers. |
Product Background |
The JD1a41b1 TCR is an engineered, high-affinity T-cell receptor that strictly recognizes the peptide fragment of KRAS G12D (VVVGADGVGK) presented on the HLA-A*11:01 only. Cluster of Differentiation 3 (CD3) is a multimeric protein complex and T cell co-receptor that plays an essential role in adaptive immune responses. These CD3 dimers bind non-covalently to the TCR to form the TCR&CD3 complex on the cell surface. |
Product Category |
Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / Nanodisc |
Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc (TCC-HM102) Specifications/Details
Protein |
KRAS G12D TCR&CD3 |
Synonyms |
KRAS G12D TCR&CD3 |
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
KRAS G12D specific TCR (JD1a41b1), CD3ε&CD3γ, CD3ε&CD3δ, and CD3ζ dimers |
Molecular Weight |
The protein has a predicted MW of 196.0 kDa. |
Activity |
Immobilized Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 17.5ng/ml determined by ELISA. The affinity constant of 0.21 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His |
Expression System |
HEK293 |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS, 200mM L-arginine (pH 7.4). |
Stability And Storage |
Valid for 6 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind OKT3, mFc Tag with an affinity constant of 0.21 nM as determined in SPR assay (Biacore T200). |
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Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with an affinity constant of 0.47 nM as determined in SPR assay (Biacore T200). |
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Immobilized Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for OKT3, mFc Tag with the EC50 of 17.5ng/ml determined by ELISA. |
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Immobilized Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag with the EC50 of 0.53ug/ml determined by ELISA. |
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Loaded Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind OKT3, mFc Tag with an affinity constant of 0.90 nM as determined in BLI assay (Gator® Prime). |
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Loaded Biotinylated Human KRAS G12D TCR&CD3 Complex Nanodisc, His Tag on Streptavidin-Biosensor can bind Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with an affinity constant of 2.60 nM as determined in BLI assay (Gator® Prime). |
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