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Human SIRP Beta/CD172b Protein

Product Info
Source

Recombinant Human SIRP Beta/CD172b Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu30-Ala369.[Accession | O00241]

Molecular Weight

The protein has a predicted MW of 40 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human SIRP Beta on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

SEC-HPLC

The purity of Human SIRP Beta is greater than 95% as determined by SEC-HPLC.

Background

SIRP beta 1 is a type I transmembrane protein belonging to the SIRP family within the Ig superfamily. Members of this family are characterized by an extracellular region containing a V-set Ig domain containing a J-like sequence and two C1-set Ig domains.SIRP beta 1 is immunoglobulin-like cell surface receptor involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Participates also in the recruitment of tyrosine kinase SYK. Triggers activation of myeloid cells when associated with TYROBP.

Synonyms

CD172 antigen-like family member B; CD172b antigen; CD172b; DKFZp686A05192; FLJ26614; signal-regulatory protein beta 1; signal-regulatory protein beta-1; SIRP beta 1; SIRPB1; SIRP-beta-1 isoform 3; SIRP-BETA-1

References

(1)Takizawa H , Manz M G . Macrophage tolerance: CD47–SIRP-α–mediated signals matter[J]. Nature Immunology, 2007, 8(12):1287-1289.

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