T7 RNA Polymerase (T7RNAP) is a protein encoded by phage T7 DNA recombinantly expressed in Escherichia coli. T7RNAP, a DNA-dependent 5'→3' RNA polymerase, highly specifically recognizes the T7 promoter sequence (5'-TAATACGACTCACTATAG-3'). The template is single-stranded or double-stranded DNA containing the T7 promoter sequence. T7RNAP synthesizes an RNA strand using NTPs, complementary to the DNA template, downstream of the promoter.
GMP-Grade T7 RNA Polymerase
FDA Drug Master Files #037660
Designed for commercial-scale mRNA manufacturing.
Inventory in stock
We engineered our T7 RNA Polymerase using Structure Aided Multiplex Screening (SAMS), our unique innovative functional recombinant protein engineering platform. It has also undergone optimization of the E. coli expression system, purification process, and buffer formulation. We've designed our T7 to produce low error rates, high efficiency, and high processivity. We've optimized the enzyme for yield and purity, making it both practical and affordable for large-scale production of mRNA / mRNA synthesis.
We manufacture our T7 RNA Polymerase in our cGMP compliant facility. Moreover, we've submitted our GMP T7RNAP to the FDA Drug Master Files (DMF #037660). Customizable regulatory support documentation including batch production records, stability testing, CoA, etc. is available.
Customizable GMP Documentation Package:
→ Melamine Statement
→ TSE/BSE Statement
→ Nitrosamine Statement
→ DMF Filing
Quality Release Specifications
Figure 1. After synthesizing mRNA in an IVT reaction, we assessed RNA transcript yield using nanodrop and Agilent 4150 tapestation. We assessed purity using capillary electrophoresis (CE). Overall, yield and purity of our T7RNAP was comparable to leading suppliers.
Low dsRNA in transcripts
Figure 2. dsRNA measured after in vitro transcription using J2-based ELISA. KACTUS T7 has low dsRNA contamination comparable or superior to leading competitors.
High yield for in vitro transcription
Figure 3. After synthesis of mRNA, in vitro transcription yield of T7 RNA Polymerase measured with Agilent 4150 Tapestation. KACTUS T7 had 65% higher yield than leading supplier.
Consistent activity across batches
Figure 4. Detection of T7 RNA Polymerase by molecular beacon. We analyzed three batches of T7 RNA Polymerase and found similar reaction kinetics across all batches.
Request a Sample
Contact us at email@example.com to inquire about a sample of our GMP T7 RNA Polymerase. Alternatively, submit the sample request form below.
To view product information or place an order online, please visit the GMP-Grade T7 RNA Polymerase product page. To order via PO, you may send your order information directly to firstname.lastname@example.org.
Our T7 RNA Polymerase is available in routine bulk supply for large-scale mRNA manufacturing. To request a bulk quote, please contact email@example.com or click below to submit a bulk quote request.
Get in Touch
T7 RNA Polymerase: FAQs
How to Order
You can order online directly by visiting the product page or contact us at firstname.lastname@example.org.
To request a test sample, submit this form.
As catalog products, we offer 1KU / 50KU / 1MU sizes. To request a bulk quote, submit this form or contact us at email@example.com.
Yes, we ship our GMP T7RNAP internationally. To place an order, visit the product page or contact us at firstname.lastname@example.org.
Visit the product page to find the product information, datasheet, MSDS, etc.
Please find the quality control specifications on this page.
Yes, our T7 RNA Polymerase can be used for linear mRNA, circular RNA, self amplifying RNA, and more. To inquire further, please contact us at email@example.com.
Yes, the T7 RNA Polymerase includes a transcription buffer that our team has highly optimized for this enzyme.
Yes, please find the recommended protocol listed in the T7 RNA Polymerase datasheet. For additional support on your IVT reaction, please contact us at firstname.lastname@example.org.
Do you still have questions regarding T7 RNA Polymerase? Contact us at email@example.com.