Product Description |
RNase R is an E. coli exoribonuclease that exhibits 3’-to- 5’ exonuclease activity, efficiently digesting nearly all linear RNA species but does not digest lariat or circular RNA structures or double-stranded RNA with 3 ́ overhangs shorter than seven nucleotides. Most cellular RNAs will be digested completely by RNase R, with the exception of tRNAs, 5S RNA, and intron lariats. RNase R is often used in alternative splicing for the isolation of circular or lariat RNA from a mixture of total RNA. |
Product Category |
Enzyme |
Synonyms |
Ribonuclease R |
Applications |
Alternative splicing and gene expression studies Intron cDNA production Intronic screening of cDNA libraries |
Concentration |
20U/µL |
Unit Definition |
One unit is defined as the amount of RNase R that converts 1 μg of poly(A) into acid-soluble nucleotides in 10 minutes at 37°C. |
Source |
E. Coli |
Biotinylated |
no |
Form |
Liquid |
Shipping |
Shipped with dry ice |
Stability And Storage |
-20±5°C for 24 months. Avoid repeated freeze-thaw cycles. |
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RNase R digests linear RNA. |
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RNase R does not digest circular RNA. |
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