Pyrophosphatase, Inorganic, GMP Grade (GMP-PYR-YE101)
Catalog: GMP-PYR-YE101
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| Product Description | Pyrophosphatase hydrolyzes inorganic pyrophosphate generated during in vitro transcription reactions. By removing unwanted pyrophosphate, the equilibrium is shifted to the product side increasing RNA yield. A PH 7.0 is ideal for optimal performance and the cofactor Mg2+ is required for enzymatic activity. Expressed in an E.coli strain with the pyrophosphatase gene from yeast. |
| Product Category | GMP Enzyme |
| Application | In vitro transcription (IVT) Optimal PH 7.0 Cofactor Mg2+ is required for enzymatic activity |
| Concentration | 0.1 U/UL |
| Unit Definition | One unit is the amount of enzyme that will generate 1μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 100 mM Tris-HCl, [pH 7.2], 2 mM MgCl2 and 2 mM PPi in a reaction volume of 0.5 ml). |
| Molecular Weight | 63 kD |
| Express System | E.coli |
| Quality Statement | Manufactured to the GMP guidelines Antibiotic-free and animal-free production Suitable for manufacturing of mRNA therapeutics and vaccines |
| QC Standard | Activity: >=126 U/ml
Purity: >=95% Residual Endonuclease: Negative Residual Exonuclease: Negative Residual RNase: Negative Residual Protease: Negative Endotoxin: <=10 EU/ml Residual Host Cell DNA: <=100 pg/MG Residual Host Protein: <=50 ng/MG Bioburden: <=1 CFU/10 ml |
| Form | Liquid |
| Shipping | Shipped with blue ice |
| Stability And Storage | -20°C for 12 months. Avoid repeated freeze-thaw cycles. |
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Fig.1 The addition of inorganic pyrophosphatase to IVT reactions can effectively increase mRNA yield, lane 1 without inorgancic pyrophosphatase, while lane 2 was added inorganic pyrophosphatase. |
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