Pyrophosphatase, Inorganic, GMP Grade (GMP-PYR-YE101)

  • Quality Statement:
    Manufactured to the GMP guidelines

    Antibiotic-free and animal-free production

    Suitable for manufacturing of mRNA therapeutics and vaccines
  • Express System: E.coli

Catalog: GMP-PYR-YE101

$600.00
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Product Description Pyrophosphatase hydrolyzes inorganic pyrophosphate generated during in vitro transcription reactions. By removing unwanted pyrophosphate, the equilibrium is shifted to the product side increasing RNA yield.
A PH 7.0 is ideal for optimal performance and the cofactor Mg2+ is required for enzymatic activity.
Expressed in an E.coli strain with the pyrophosphatase gene from yeast.
Product Category GMP Enzyme
Application In vitro transcription (IVT)
Optimal PH 7.0
Cofactor Mg2+ is required for enzymatic activity
Concentration 0.1 U/UL
Unit Definition One unit is the amount of enzyme that will generate 1μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 100 mM Tris-HCl, [pH 7.2], 2 mM MgCl2 and 2 mM PPi in a reaction volume of 0.5 ml).
Molecular Weight 63 kD
Express System E.coli
Quality Statement
Manufactured to the GMP guidelines

Antibiotic-free and animal-free production

Suitable for manufacturing of mRNA therapeutics and vaccines
QC Standard Activity: >=126 U/ml
Purity: >=95%
Residual Endonuclease: Negative
Residual Exonuclease: Negative
Residual RNase: Negative
Residual Protease: Negative
Endotoxin: <=10 EU/ml
Residual Host Cell DNA: <=100 pg/MG
Residual Host Protein: <=50 ng/MG
Bioburden: <=1 CFU/10 ml
Form Liquid
Shipping Shipped with blue ice
Stability And Storage -20°C for 12 months. Avoid repeated freeze-thaw cycles.
Fig.1 The addition of inorganic pyrophosphatase to IVT reactions can effectively increase mRNA yield, lane 1 without inorgancic pyrophosphatase, while lane 2 was added inorganic pyrophosphatase.

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