Pyrophosphatase, Inorganic, GMP Grade (GMP-PYR-YE101)
|Product Description||Pyrophosphatase hydrolyzes inorganic pyrophosphate generated during in vitro transcription reactions. By removing unwanted pyrophosphate, the equilibrium is shifted to the product side increasing RNA yield.
A PH 7.0 is ideal for optimal performance and the cofactor Mg2+ is required for enzymatic activity.
Expressed in an E.coli strain with the pyrophosphatase gene from yeast.
|Product Category||GMP Enzyme|
|Application||In vitro transcription (IVT)
Optimal PH 7.0
Cofactor Mg2+ is required for enzymatic activity
|Unit Definition||One unit is the amount of enzyme that will generate 1μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 100 mM Tris-HCl, [pH 7.2], 2 mM MgCl2 and 2 mM PPi in a reaction volume of 0.5 ml).|
|Molecular Weight||63 kD|
Manufactured to the GMP guidelines Antibiotic-free and animal-free production Suitable for manufacturing of mRNA therapeutics and vaccines
|QC Standard||Activity: >=126 U/ml
Residual Endonuclease: Negative
Residual Exonuclease: Negative
Residual RNase: Negative
Residual Protease: Negative
Endotoxin: <=10 EU/ml
Residual Host Cell DNA: <=100 pg/MG
Residual Host Protein: <=50 ng/MG
Bioburden: <=1 CFU/10 ml
|Shipping||Shipped with blue ice|
|Stability And Storage||-20°C for 12 months. Avoid repeated freeze-thaw cycles.|