Pyrophosphatase, Inorganic, GMP-Grade (GMP-PYR-YE101), DMF #038030

Catalog: GMP-PYR-YE101


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Product Description Pyrophosphatase hydrolyzes inorganic pyrophosphate generated during in vitro transcription reactions. By removing unwanted pyrophosphate, the equilibrium is shifted to the product side increasing RNA yield.

NOTE: A PH 7.0 is ideal for optimal performance and the cofactor Mg2+ is required for enzymatic activity.

This product has been filed with the FDA Drug Master Files (DMF) and is assigned DMF #038030.
Application In vitro transcription (IVT)
Concentration 0.1U/µL
Unit Definition One unit is the amount of enzyme that will generate 1µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 100 mM Tris-HCl, [pH 7.2], 2 mM MgCl2 and 2 mM PPi in a reaction volume of 0.5 mL).
Source Expressed in an E.coli strain with the pyrophosphatase gene from yeast.
Molecular Weight 63 kD
Quality Standards Activity (Enzyme Catalytic Efficiency Determination): ≥ 126 U/mL
Purity (SEC-HPLC): ≥ 95%
Residual Endonuclease: Negative
Residual Exonuclease: Negative
Residual RNase: Negative
Residual Protease: Negative
Endotoxin: ≤ 10 EU/mL
Residual Host Cell DNA: ≤ 100pg/mg
Residual Host Protein: ≤ 50ng/mg
Bioburden: ≤ 1 CFU/10mL
Form Liquid
Shipping Shipped with blue ice
Stability And Storage -20±5°C for 24 months. Avoid repeated freeze-thaw cycles.
Figure 1. The addition of inorganic pyrophosphatase to IVT reactions can effectively increase mRNA yield. Lane 1 was without inorganic pyrophosphatase, while lane 2 had added inorganic pyrophosphatase.

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