Pyrophosphatase, Inorganic, GMP-Grade (GMP-PYR-YE101), DMF #038030
|Product Description||Pyrophosphatase hydrolyzes inorganic pyrophosphate generated during in vitro transcription reactions. By removing unwanted pyrophosphate, the equilibrium is shifted to the product side increasing RNA yield.
NOTE: A PH 7.0 is ideal for optimal performance and the cofactor Mg2+ is required for enzymatic activity.
This product has been filed with the FDA Drug Master Files (DMF) and is assigned DMF #038030.
|Application||In vitro transcription (IVT)|
|Unit Definition||One unit is the amount of enzyme that will generate 1µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 100 mM Tris-HCl, [pH 7.2], 2 mM MgCl2 and 2 mM PPi in a reaction volume of 0.5 mL).|
|Source||Expressed in an E.coli strain with the pyrophosphatase gene from yeast.|
|Molecular Weight||63 kD|
|Quality Standards||Activity (Enzyme Catalytic Efficiency Determination): ≥ 126 U/mL
Purity (SEC-HPLC): ≥ 95%
Residual Endonuclease: Negative
Residual Exonuclease: Negative
Residual RNase: Negative
Residual Protease: Negative
Endotoxin: ≤ 10 EU/mL
Residual Host Cell DNA: ≤ 100pg/mg
Residual Host Protein: ≤ 50ng/mg
Bioburden: ≤ 1 CFU/10mL
|Shipping||Shipped with blue ice|
|Stability And Storage||-20±5°C for 24 months. Avoid repeated freeze-thaw cycles.|