Product Description |
Pyrophosphatase hydrolyzes inorganic pyrophosphate generated during in vitro transcription reactions. By removing unwanted pyrophosphate, the equilibrium is shifted to the product side increasing RNA yield. NOTE: A PH 7.0 is ideal for optimal performance and the cofactor Mg2+ is required for enzymatic activity. This product has been filed with the FDA Drug Master Files (DMF) and is assigned DMF #038030. |
Applications |
In vitro transcription (IVT) |
Concentration |
0.1U/µL |
Unit Definition |
One unit is the amount of enzyme that will generate 1µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 100 mM Tris-HCl, [pH 7.2], 2 mM MgCl2 and 2 mM PPi in a reaction volume of 0.5 mL). |
Source |
Expressed in an E.coli strain with the pyrophosphatase gene from yeast. |
Biotinylated |
no |
Molecular Weight |
33.4 kD |
Quality Standards |
Activity (Enzyme Catalytic Efficiency Determination): ≥ 126 U/mL
Purity (SEC-HPLC): ≥ 95% Residual Nickel Salt: ≤ 10 ppm Residual Heavy Metal: ≤ 10ppm Residual RNase: Negative Residual Protease: Negative Endotoxin: ≤ 1 EU/mL Residual Host Cell DNA: ≤ 100pg/mg Residual Host Protein: ≤ 20ng/mg Bioburden: ≤ 1 CFU/10mL |
Form |
Liquid |
Shipping |
Shipped with blue ice |
Stability And Storage |
-20±5°C for 24 months. Avoid repeated freeze-thaw cycles. |
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Figure 1. The addition of inorganic pyrophosphatase to IVT reactions can effectively increase mRNA yield. Lane 1 was without inorganic pyrophosphatase, while lane 2 had added inorganic pyrophosphatase. |
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