Pyrophosphatase, Inorganic, GMP-Grade (GMP-PYR-YE101), DMF #038030
Catalog: GMP-PYR-YE101
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| Product Description | Pyrophosphatase hydrolyzes inorganic pyrophosphate generated during in vitro transcription reactions. By removing unwanted pyrophosphate, the equilibrium is shifted to the product side increasing RNA yield. NOTE: A PH 7.0 is ideal for optimal performance and the cofactor Mg2+ is required for enzymatic activity. This product has been filed with the FDA Drug Master Files (DMF) and is assigned DMF #038030. |
| Application | In vitro transcription (IVT) |
| Concentration | 0.1U/µL |
| Unit Definition | One unit is the amount of enzyme that will generate 1µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 100 mM Tris-HCl, [pH 7.2], 2 mM MgCl2 and 2 mM PPi in a reaction volume of 0.5 mL). |
| Source | Expressed in an E.coli strain with the pyrophosphatase gene from yeast. |
| Molecular Weight | 33.4 kD |
| Quality Standards | Activity (Enzyme Catalytic Efficiency Determination): ≥ 126 U/mL
Purity (SEC-HPLC): ≥ 95% Residual Nickel Salt: ≤ 10 ppm Residual Heavy Metal: ≤ 10ppm Residual RNase: Negative Residual Protease: Negative Endotoxin: ≤ 1 EU/mL Residual Host Cell DNA: ≤ 100pg/mg Residual Host Protein: ≤ 20ng/mg Bioburden: ≤ 1 CFU/10mL |
| Form | Liquid |
| Shipping | Shipped with blue ice |
| Stability And Storage | -20±5°C for 24 months. Avoid repeated freeze-thaw cycles. |
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Figure 1. The addition of inorganic pyrophosphatase to IVT reactions can effectively increase mRNA yield. Lane 1 was without inorganic pyrophosphatase, while lane 2 had added inorganic pyrophosphatase. |
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