Product Description |
T4 RNA Ligase II, also known as T4 Rnl2 (gp24.1), is an ATP-dependent RNA ligase catalyzing the inter- and intramolecular RNA strand joining activity via phosphodiester bond formation. Unlike T4 RNA Ligase I, T4 RNA Ligase II is much more active in joining nicks on double-stranded RNA (dsRNA) than joining the ends of single-stranded RNA. The enzyme can also ligate the 3´ OH of RNA to the 5´ phosphate of DNA in a double-stranded structure. The enzyme requires an adjacent 5´ phosphate and 3´ OH for ligation. |
Product Category |
Enzyme |
Synonyms |
T4 RNA Ligase II; T4 RNA Ligase 2; dsRNA Ligase; T4 Rnl2; Rnl2; T4Rnl2 |
Applications |
Joining nicks on double-stranded RNA Joining 5’ phosphate of oligodeoxyribonucleotides and 3’OH of oligoribonucleotides |
Concentration |
10U/µL |
Unit Definition |
One unit is defined as the amount of enzyme required to ligate 0.4 µg of an equimolar mix of a 23-mer and 17-mer RNAs in a total reaction volume of 20 µL in 30 minutes at 37°C. |
Source |
E. coli |
Form |
Liquid |
Shipping |
Transport on dry ice. |
Formulation |
Liquid |
Stability And Storage |
Store at -20°C ± 5°C for up to 24 months. Avoid repeated freeze/thaw cycles. |
|
|
Ligation of a 22mer RNA substrate using T4 RNA ligase II, followed by Urea page gel analysis. |
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