Product Description |
T4 RNA Ligase II, also known as T4 Rnl2 (gp24.1), is an ATP-dependent RNA ligase catalyzing the inter- and intramolecular RNA strand joining activity via phosphodiester bond formation. Unlike T4 RNA Ligase I, T4 RNA Ligase II is much more active in joining nicks on double-stranded RNA (dsRNA) than joining the ends of single-stranded RNA. The enzyme can also ligate the 3´ OH of RNA to the 5´ phosphate of DNA in a double-stranded structure. The enzyme requires an adjacent 5´ phosphate and 3´ OH for ligation. |