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Human SIRP Gamma/CD172g Protein

Product Info
Source

Recombinant Human SIRP Gamma/CD172g Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Glu29-Pro360.[Accession | Q9P1W8]

Molecular Weight

The protein has a predicted MW of 39.7 kDa. Due to glycosylation, the protein migrates to 47-49 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by Tris-Bis PAGE
> 95% as determined by SEC-HPLC

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage

The product should be stored at -70℃ or -20℃.

Assay Data
Tris-Bis PAGE

Human SIRP Gamma on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%..

SEC-HPLC

The purity of Human SIRP Gamma is greater than 95% as determined by SEC-HPLC.

ELISA Data

Immobilized Human SIRP Gamma at 0.5μg/ml (100μl/Well). Dose response curve for Anti-SIRP Ab., hFc Tag with the EC50 of 12.8ng/ml determined by ELISA.

Background

Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily.SIRP gamma is probable immunoglobulin-like cell surface receptor. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation.

Synonyms

CD172g antigen; CD172g; FLJ42230; signal-regulatory protein beta 2; Signal-regulatory protein beta-2; signal-regulatory protein gamma; SIRP beta 2; SIRP gamma; SIRP-B2; SIRPB2CD172 antigen-like family member B; SIRP-beta-2; SIRPG; SIRPgamma; SIRP-gammabA77C3.

References

(1)Piccio, L. Adhesion of human T cells to antigen-presenting cells through SIRP beta2-CD47 interaction costimulates T-cell proliferation[J]. Blood, 2005, 105(6):2421-2427.

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