Product Description |
Recombinant Biotinylated Human Activin A Protein is expressed from HEK293 with Avi tag at the C-terminus; It contains Gly311-Ser426. |
Product Background |
Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
Activin A |
Synonyms |
INHBA; Activin A; |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly311-Ser426 |
Molecular Weight |
The protein has a predicted MW of 14.78 kDa. Due to glycosylation, the protein migrates to 15-18 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Activin RIIB, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Activin A, Avi Tag with the EC50 of 3.0 ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-Avi |
Expression System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in 4 mM HCL. |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human Activin RIIB, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Activin A, Avi Tag with the EC50 of 3.0 ng/ml determined by ELISA (QC Test). |
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Biotinylated Human Activin A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Serial dilutions of Anti-Activin RIIB Antibody were added into Human Activin RIIB, His Tag : Biotinylated Human Activin A, Avi Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.14ug/ml. |
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