Biotinylated Human Activin A Protein (ACV-HM401B)
  • Express System: HEK293
  • Product Tag: C-Avi
  • Purity: >95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human Activin RIIB, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Activin A, Avi Tag with the EC50 of 3.0 ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly311-Ser426
Catalog #ACV-HM401B
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Product Description
 Recombinant Biotinylated Human Activin A Protein is expressed from HEK293 with Avi tag at the C-terminus; It contains Gly311-Ser426.
Product Background
 Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression.
Product Category
 Recombinant Protein
Target
 Activin A
Synonyms
 INHBA; Activin A;
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gly311-Ser426
Molecular Weight
 The protein has a predicted MW of 14.78 kDa. Due to glycosylation, the protein migrates to 15-18 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Activin RIIB, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Activin A, Avi Tag with the EC50 of 3.0 ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-Avi
Express System
 HEK293
Purity
 >95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 4 mM HCL. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Activin RIIB, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Activin A, Avi Tag with the EC50 of 3.0 ng/ml determined by ELISA.
Biotinylated Human Activin A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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