ELISA binding curve showing interaction between biotinylated human Activin A (Avi tag) and human Activin RIIB His-tagged protein, with absorbance plotted against ligand concentration.
  • Express System: HEK293
  • Product Tag: C-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi Tag with the EC50 of 3.0 ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gly311-Ser426
Catalog #ACV-HM401B
Regular price $660.00
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Product Description

Recombinant Biotinylated Human/Mouse/Rat Activin A Protein is expressed from HEK293 with Avi tag at the C-terminus. It contains Gly311-Ser426.

Product Background

Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

Activin A

Synonyms

INHBA; Activin A;

Accession

Species

Human/Mouse/Rat

Biotinylated

yes

Amino Acid Range

Gly311-Ser426

Molecular Weight

The protein has a predicted MW of 14.78 kDa. Due to glycosylation, the protein migrates to 15-18 kDa under reduced (R) condition, and 23-30 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.

Activity

Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi Tag with the EC50 of 3.0 ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 4 mM HCL. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi Tag with the EC50 of 3.0 ng/ml determined by ELISA (QC Test). Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi Tag with the EC50 of 3.0 ng/ml determined by ELISA (QC Test).
Biotinylated Human/Mouse/Rat Activin A on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%. Biotinylated Human/Mouse/Rat Activin A on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.
Serial dilutions of Bimagrumab were added into Human Activin RIIB, His Tag : Biotinylated Human/Mouse/Rat Activin A, Avi Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.14ug/ml. Serial dilutions of Bimagrumab were added into Human Activin RIIB, His Tag : Biotinylated Human/Mouse/Rat Activin A, Avi Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.14ug/ml.

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support@kactusbio.us

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