Name: Biotinylated Human/Mouse/Rat Activin A Protein (ACV-HM401B)
Brand: KactusBio
SKU: ACV-HM401B-100UG
Price: 660.0 USD
Availability: InStock

ELISA binding curve showing interaction between biotinylated human Activin A (Avi tag) and human Activin RIIB His-tagged protein, with absorbance plotted against ligand concentration.

Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi Tag with the EC50 of 3.0 ng/ml determined by ELISA (QC Test).

Biotinylated Human/Mouse/Rat Activin A on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

Serial dilutions of Bimagrumab were added into Human Activin RIIB, His Tag : Biotinylated Human/Mouse/Rat Activin A, Avi Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.14ug/ml.

Express System: HEK293
Product Tag: C-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi Tag with the EC50 of 3.0 ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Gly311-Ser426

Catalog #ACV-HM401B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human/Mouse/Rat Activin A Protein is expressed from HEK293 with Avi tag at the C-terminus. It contains Gly311-Ser426.
Product Background	Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	Activin A
Synonyms	INHBA; Activin A;
Accession	P08476
Species	Human/Mouse/Rat
Biotinylated	yes
Amino Acid Range	Gly311-Ser426
Molecular Weight	The protein has a predicted MW of 14.78 kDa. Due to glycosylation, the protein migrates to 15-18 kDa under reduced (R) condition, and 23-30 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Activity	Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi Tag with the EC50 of 3.0 ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 4 mM HCL. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human/Cynomolgus Activin RIIB, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi Tag with the EC50 of 3.0 ng/ml determined by ELISA (QC Test).
	Biotinylated Human/Mouse/Rat Activin A on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.
	Serial dilutions of Bimagrumab were added into Human Activin RIIB, His Tag : Biotinylated Human/Mouse/Rat Activin A, Avi Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.14ug/ml.