Biotinylated Human Activin A Protein (ACV-HM401B)

Immobilized Human/Cynomolgus Activin RIIB, His Tag (Cat. ARB-HM12B) at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi Tag with the EC50 of 3.2 ng/ml determined by ELISA.

Immobilized Human/Cynomolgus Activin RIIA, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi Tag with the EC50 of 10.4ng/ml determined by ELISA.

Biotinylated Human/Mouse/Rat Activin A on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.

Serial dilutions of Bimagrumab were added into Human/Cynomolgus Activin RIIB, His Tag : Biotinylated Human/Mouse/Rat Activin A, Avi Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.14ug/ml.

Loaded Biotinylated Human Activin A, Avi Tag on Streptavidin-Biosensor can bind Human Activin RIIB, His Tag with an affinity constant of 30.40 nM as determined in BLI assay.

Express System: HEK293
Product Tag: C-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human/Cynomolgus Activin RIIB, His Tag (Cat. ARB-HM12B) at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi Tag with the EC50 of 3.2 ng/ml determined by ELISA.
Amino Acid Range: Gly311-Ser426

Catalog #ACV-HM401B

Price

$660⁰⁰

Size

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Biotinylated Human/Mouse/Rat Activin A Protein (ACV-HM401B) Summary

Product Description

Recombinant Biotinylated Human/Mouse/Rat Activin A Protein is expressed from HEK293 with Avi tag at the C-terminus. It contains Gly311-Ser426.

Product Background

Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression.

Biotinylated Human/Mouse/Rat Activin A Protein (ACV-HM401B) Specifications/Details

Protein	Activin A
Synonyms	INHBA; Activin A;
Accession	P08476
Species	Human/Mouse/Rat
Biotinylated	yes
Amino Acid Range	Gly311-Ser426
Molecular Weight	The protein has a predicted MW of 14.78 kDa. Due to glycosylation, the protein migrates to 15-18 kDa under reduced (R) condition, and 23-30 kDa under Non reduced (N) condition based on Bis-Tris PAGE result.
Activity	Immobilized Human/Cynomolgus Activin RIIB, His Tag (Cat. ARB-HM12B) at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi Tag with the EC50 of 3.2 ng/ml determined by ELISA.
Product Tag	C-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in 4mM HCL.
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human/Cynomolgus Activin RIIB, His Tag (Cat. ARB-HM12B) at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi Tag with the EC50 of 3.2 ng/ml determined by ELISA.
	Immobilized Human/Cynomolgus Activin RIIA, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Mouse/Rat Activin A, Avi Tag with the EC50 of 10.4ng/ml determined by ELISA.
	Biotinylated Human/Mouse/Rat Activin A on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.
	Serial dilutions of Bimagrumab were added into Human/Cynomolgus Activin RIIB, His Tag : Biotinylated Human/Mouse/Rat Activin A, Avi Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.14ug/ml.
	Loaded Biotinylated Human Activin A, Avi Tag on Streptavidin-Biosensor can bind Human Activin RIIB, His Tag with an affinity constant of 30.40 nM as determined in BLI assay.