Biotinylated Human Activin A Protein (ACV-HM401B)
Catalog: ACV-HM401B
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| Product Description | Recombinant Biotinylated Human Activin A Protein is expressed from HEK293 with Avi tag at the C-terminus.; It contains Gly311-Ser426. |
Product Background |
Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression. |
Product Category |
Recombinant Protein |
Target |
Activin A |
Gene/Protein ID |
P08476 |
Species |
Human |
Exact Sequence |
Gly311-Ser426 |
| Molecular Weight | The protein has a predicted MW of 14.78 kDa. Due to glycosylation, the protein migrates to 15-18 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human Activin RIIB, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Activin A, Avi Tag with the EC50 of 3.0 ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE |
| Endotoxin | Less than 1 EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in 4 mM HCL. Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 4mM HCL. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human Activin RIIB, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human Activin A, Avi Tag with the EC50 of 3.0 ng/ml determined by ELISA. |
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Biotinylated Human Activin A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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