Name: Mouse GDF15 Protein (GDF-ME115)
Brand: KactusBio
SKU: GDF-ME115-100UG
Price: 315.0 USD
Availability: InStock

Express System: E.coli
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Mouse GDF15, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His-Avi Tag with the EC50 of 1.23ug/ml determined by ELISA (QC Test). The affinity constant of 0.34 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ser189-Ala303

Catalog #GDF-ME115

Size

100UG

500UG

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Product Description	Recombinant Mouse GDF15 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ser189-Ala303.
Product Background	Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance.
Product Category	Recombinant Protein / Antibody discovery

Protein	GDF15
Synonyms	GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β
Accession	Q9Z0J7
Species	Mouse
Biotinylated	no
Amino Acid Range	Ser189-Ala303
Molecular Weight	The protein has a predicted MW of 13.78 kDa. The protein migrates to 15-16 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Mouse GDF15, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His-Avi Tag with the EC50 of 1.23ug/ml determined by ELISA (QC Test). The affinity constant of 0.34 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Mouse GFRAL, His Tag immobilized on CM5 Chip can bind Mouse GDF15, His Tag with an affinity constant of 0.34 nM as determined in SPR assay (Biacore T200).
	Immobilized Mouse GDF15, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His-Avi Tag with the EC50 of 1.23ug/ml determined by ELISA (QC Test).
	Mouse GDF15 on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.