Mouse GDF15 Protein (GDF-ME115)

  • Express System: E.coli
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Mouse GDF15, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.68μg/ml determined by ELISA. The affinity constant of 0.34 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ser189-Ala303

Catalog: GDF-ME115

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Product Description Recombinant Mouse GDF15 Protein is expressed from E.coli with His tag at the N-terminal.It contains Ser189-Ala303.
Product Category Recombinant Protein
Target GDF15
Gene/Protein ID Q9Z0J7
Species Mouse
Exact Sequence Ser189-Ala303
Molecular Weight The protein has a predicted MW of 13.78 kDa. The protein migrates to 15-16 kDa based on Tris-Bis PAGE result.
Activity Immobilized Mouse GDF15, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.68μg/ml determined by ELISA. The affinity constant of 0.34 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-His
Express System E.coli
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in 50mM HAc (pH 2.9).
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse GFRAL, His Tag immobilized on CM5 Chip can bind Mouse GDF15, His Tag with an affinity constant of 0.34 nM as determined in SPR assay (Biacore T200).
Immobilized Mouse GDF15, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.68μg/ml determined by ELISA.
Mouse GDF15 on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

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