Mouse GDF15 Protein (GDF-ME115)

Express System: E.coli
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Mouse GDF15, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.25ug/ml determined by ELISA (QC Test). The affinity constant of 0.34 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ser189-Ala303

Catalog: GDF-ME115

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Product Description	Recombinant Mouse GDF15 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ser189-Ala303.
Product Background	Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance.
Product Category	Recombinant Protein

Target	GDF15
Gene/Protein ID	Q9Z0J7
Species	Mouse
Exact Sequence	Ser189-Ala303
Molecular Weight	The protein has a predicted MW of 13.78 kDa. The protein migrates to 15-16 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse GDF15, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.25ug/ml determined by ELISA (QC Test). The affinity constant of 0.34 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Mouse GFRAL, His Tag immobilized on CM5 Chip can bind Mouse GDF15, His Tag with an affinity constant of 0.34 nM as determined in SPR assay (Biacore T200).
	Immobilized Mouse GDF15, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GFRAL, His Tag with the EC50 of 0.25ug/ml determined by ELISA (QC Test).
	Mouse GDF15 on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.