Biotinylated Human DLL3 Domain (311-479) Protein (DLL-HM4D1B)
Biotinylated Human DLL3 Domain (311-479) Protein (DLL-HM4D1B)
Biotinylated Human DLL3 Domain (311-479) Protein (DLL-HM4D1B)
Biotinylated Human DLL3 Domain (311-479) Protein (DLL-HM4D1B)
Biotinylated Human DLL3 Domain (311-479) Protein (DLL-HM4D1B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human DLL3 Domain (311-479), His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Val311-Ala479
Catalog #DLL-HM4D1B
Regular price $577.50
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Product Description

Recombinant Biotinylated Human DLL3 Domain (311-479) Protein is expressed from HEK293 with with His tag and Avi tag at the C-Terminus.It contains Val311-Ala479.

Product Background

Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

DLL3

Synonyms

Delta3; DLL3; Pudgy; SCDO1; SCDO1delta3

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Val311-Ala479

Molecular Weight

The protein has a predicted MW of 21.20 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human DLL3 Domain (311-479), His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human DLL3 Domain (311-479), His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. Immobilized Biotinylated Human DLL3 Domain (311-479), His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
Biotinylated Human DLL3 Domain (311-479) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human DLL3 Domain (311-479) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Anti-DLL3 Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human DLL3 (311-479), His-Avi Tag with an affinity constant of 0.22 nM as determined in BLI assay (Gator® Prime). Loaded Anti-DLL3 Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human DLL3 (311-479), His-Avi Tag with an affinity constant of 0.22 nM as determined in BLI assay (Gator® Prime).
The purity of Biotinylated Human DLL3 Domain (311-479) is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human DLL3 Domain (311-479) is greater than 95% as determined by SEC-HPLC.

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