Product Description |
Recombinant Biotinylated Human DLL3 Domain (311-479) Protein is expressed from HEK293 with with His tag and Avi tag at the C-Terminus.It contains Val311-Ala479. |
Product Background |
Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity). |
Product Category |
Recombinant Protein |
Target |
DLL3 Domain (311-479) |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Val311-Ala479 |
Molecular Weight |
The protein has a predicted MW of 21.20 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human DLL3 Domain (311-479), His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human DLL3 Domain (311-479), His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA (QC Test). |
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|
Biotinylated Human DLL3 Domain (311-479) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human DLL3 Domain (311-479) is greater than 95% as determined by SEC-HPLC. |
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