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Biotinylated Human Siglec-3/CD33 Protein (CD3-HM433B) Summary
Product Description |
Recombinant Biotinylated Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp18-His259. |
Product Background |
CD33 (siglec-3), a well-known target in leukemia therapy, is an inhibitory sialoadhesin expressed in human leukocytes of the myeloid lineage and some lymphoid subsets, including NK cells. CD33 is the smallest siglec member. It preferentially binds to α2-6- and α2-3-sialylated glycans and strongly binds to sialylated ligands on leukemic cell lines. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets | ADC targets |
Biotinylated Human Siglec-3/CD33 Protein (CD3-HM433B) Specifications/Details
Protein |
Siglec-3/CD33 |
Synonyms |
CD33 molecule; CD33; FLJ00391; gp67; Siglec3; Siglec-3; p67 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Asp18-His259 |
Molecular Weight |
The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human Siglec-3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22um filtered solution in 50mM MES, 500mM NaCl, 200mM L-arginine (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human Siglec-3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. |
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Biotinylated Human Siglec-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human Siglec-3 is greater than 95% as determined by SEC-HPLC. |
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