||The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
||Immobilized Biotinylated Human Siglec-3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
||> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
||Less than 1EU per ug by the LAL method.