Biotinylated Human Siglec-3/CD33 Protein (CD3-HM433B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-Siglec-3 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-3, His Tag with the EC50 of 40.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp18-His259

Catalog: CD3-HM433B

$657.00
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Product Description Recombinant Biotinylated Human Siglec-3/CD33 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Asp18-His259.
Product Category Recombinant Protein
Target Siglec-3/CD33
Gene/Protein ID P20138-1
Species Human
Exact Sequence Asp18-His259
Molecular Weight The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-Siglec-3 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-3, His Tag with the EC50 of 40.9ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22μm filtered solution in 20mM PB, 500mM NaCl (pH 8.0). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
Immobilized Anti-Siglec-3 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-3, His Tag with the EC50 of 40.9ng/ml determined by ELISA.
The purity of Biotinylated Human Siglec-3 is greater than 90% as determined by SEC-HPLC.
Biotinylated Human Siglec-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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