Biotinylated Human Siglec-3/CD33 Protein (CD3-HM433B)
Catalog: CD3-HM433B
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| Product Description | Recombinant Biotinylated Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp18-His259. |
| Product Category | Recombinant Protein |
| Target | Siglec-3/CD33 |
| Gene/Protein ID | P20138-1 |
| Species | Human |
| Exact Sequence | Asp18-His259 |
| Molecular Weight | The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Biotinylated Human Siglec-3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Liquid |
| Shipping | Shipped with dry ice. |
| Formulation | Supplied as 0.22um filtered solution in 50m MES, 500mM NaCl, 200mM L-arginine (pH 7.4). |
| Stability And Storage | Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Biotinylated Human Siglec-3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. |
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The purity of Biotinylated Human Siglec-3 is greater than 90% as determined by SEC-HPLC. |
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Biotinylated Human Siglec-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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