Biotinylated Human Siglec-3/CD33 Protein (CD3-HM433B)
|Recombinant Biotinylated Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp18-His259.
|Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.
|The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
|Immobilized Biotinylated Human Siglec-3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
|> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
|Less than 1EU per ug by the LAL method.
|Shipped with dry ice.
|Supplied as 0.22um filtered solution in 50m MES, 500mM NaCl, 200mM L-arginine (pH 7.4).
|Stability And Storage
|Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.