Biotinylated Human Siglec-3/CD33 Protein (CD3-HM433B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human Siglec-3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp18-His259

Catalog: CD3-HM433B

$657.00
Size:

Order now get it on

Product Description Recombinant Biotinylated Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp18-His259.
Product Category Recombinant Protein
Target Siglec-3/CD33
Gene/Protein ID P20138-1
Species Human
Exact Sequence Asp18-His259
Molecular Weight The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human Siglec-3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22um filtered solution in 50m MES, 500mM NaCl, 200mM L-arginine (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human Siglec-3, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
The purity of Biotinylated Human Siglec-3 is greater than 90% as determined by SEC-HPLC.
Biotinylated Human Siglec-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in