- Express System: HEK293
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Cynomolgus APOH, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 41.1ng/ml determined by ELISA (QC Test).
- Amino Acid Range: Gly20-Cys345
-
Other Available Proteins:
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Cynomolgus APOH Protein (APH-CM101) Summary
Product Description |
Recombinant Cynomolgus APOH Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Cys345. |
Product Background |
Apolipoprotein (apo)H (also known as beta 2 glycoprotein-I) is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. APOH displays a genetically determined structural polymorphism: three alleles (APOH*1, APOH*2, APOH*3) at a single locus on chromosome 17 code for different isoforms, and population studies have shown that APOH*2 is the most frequent allele. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Cynomolgus APOH Protein (APH-CM101) Specifications/Details
Protein |
APOH |
Synonyms |
Apo-H; B2GP1; B2G1; APOH; B2GPI; beta(2)GPI; BG;β(2)GPI; |
Accession |
|
Species |
Cynomolgus |
Biotinylated |
no |
Amino Acid Range |
Gly20-Cys345 |
Molecular Weight |
The protein has a predicted MW of 37.39 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Cynomolgus APOH, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 41.1ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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