Cynomolgus APOH Protein (APH-CM101)
Cynomolgus APOH Protein (APH-CM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus APOH, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 89.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly20-Cys345
Catalog #APH-CM101
Regular price $315.00
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Product Description
Recombinant Cynomolgus APOH Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Cys345.
Product Background
Apolipoprotein (apo)H (also known as beta 2 glycoprotein-I) is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. APOH displays a genetically determined structural polymorphism: three alleles (APOH*1, APOH*2, APOH*3) at a single locus on chromosome 17 code for different isoforms, and population studies have shown that APOH*2 is the most frequent allele.
Product Category
Recombinant Protein
Target
APOH
Accession
Species
Cynomolgus
Biotinylated
no
Amino Acid Range
Gly20-Cys345
Molecular Weight
The protein has a predicted MW of 37.39 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Cynomolgus APOH, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 89.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS, 100mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus APOH, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 89.8ng/ml determined by ELISA.
Cynomolgus APOH on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus APOH is greater than 95% as determined by SEC-HPLC.

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